Li H B, Chen F
Department of Botany, The University of Hong Kong, Hong Kong, China.
J Chromatogr A. 2001 Aug 3;925(1-2):109-14. doi: 10.1016/s0021-9673(01)01026-3.
A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of six diterpenoids. dihydrotanshinone I, cryptotanshinone, methylenetanshiquinone, tanshinone I, tanshinone IIA and danshenxinkun B from the Chinese medicinal plant Salvia miltiorrhiza Bunge. The crude diterpenoids were obtained by extraction with ethanol-n-hexane (1:1, v/v) from S. miltiorrhiza Bunge. Preparative HSCCC with the two-phase solvent systems A composed of n-hexane-ethanol-water (10:5.5:4.5, v/v) and B composed of n-hexane-ethanol-water (10:7:3, v/v) was successfully performed in a stepwise elution yielding six relatively pure diterpenoids from 300 mg of the crude extract in a single run. The purities of dihydrotanshinone I, cryptotanshinone, methylenetanshiquinone, tanshinone I, tanshinone IIA and danshenxinkun B were 88.1, 98.8, 97.6, 93.5, 96.8 and 94.3%, respectively.
建立了一种高速逆流色谱（HSCCC）方法，用于从中药丹参中制备分离和纯化6种二萜类化合物，即二氢丹参酮I、隐丹参酮、亚甲基丹参醌、丹参酮I、丹参酮IIA和丹参新醌B。通过用乙醇 - 正己烷（1:1，v/v）从丹参中提取得到粗二萜类化合物。采用由正己烷 - 乙醇 - 水（10:5.5:4.5，v/v）组成的两相溶剂系统A和由正己烷 - 乙醇 - 水（10:7:3，v/v）组成的两相溶剂系统B进行制备型HSCCC，通过梯度洗脱成功地从300 mg粗提取物中单次运行得到6种相对纯的二萜类化合物。二氢丹参酮I、隐丹参酮、亚甲基丹参醌、丹参酮I、丹参酮IIA和丹参新醌B的纯度分别为88.1%、98.8%、97.6%、93.5%、96.8%和94.3%。
