Nakamura Kazuki, Yoshikawa Noriko, Yamaguchi Yu, Kagota Satomi, Shinozuka Kazumasa, Kunitomo Masaru
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Life Sci. 2002 Jan 4;70(7):791-8. doi: 10.1016/s0024-3205(01)01454-0.
We characterized the metastatic ability and mortality of four different mouse melanoma cell lines, B16-F0, -F1, -F10 and -BL6. B16-F0 is the parent cell line. B16-F1 was obtained by a one-time selective procedure and B16-F10 by a ten-time selective procedure using Fidler's method. B16-BL6 derived from B16-F10 has much more invasive activity than B16-F10. To investigate the difference in mortal malignancy among B16-F0, -F1, -F10 and -BL6, we examined the survival time of syngeneic C57BL/6Cr mice intravenously inoculated with these cells. As a control, we used the C57BL/6J-embryo mouse fibroblast-like semi-normal cell line. The ability to form lung metastatic nodules in mice gradually increased in the order: B16-F0, -F1, and -F10 (=-BL6). C57BL/6J-embryo cell (1 x 10(5)/mouse)-inoculated mice survived for over 46 days. B16-F0, -F1, -F10 and -BL6 (1 x 10(5)/mouse)-inoculated mice survived 31.4+/-4.4 (7), 25.7+/-2.8 (7), 23.6+/-1.5 (7) and 25.3+/-2.3 (7) days [mean+/-S.D. (number of mice)], respectively. According to the Mann-Whitney test, the B16-F0 inoculated group versus -F1 inoculated group (P<0.05), -F0 inoculated group versus -BL6 inoculated group (P<0.05), and -F0 inoculated group versus -F10 inoculated group (P<0.01) were significantly different, but the B16-F1 group versus -F10 group, -F1 group versus -BL6 group, and -F10 group versus -BL6 group were not. These results suggest that mortal malignancy is not necessarily correlated with lung-colonizing potential and even only one-time selected B16-F0 mouse melanoma cells are useful as an experimental metastatic model in vivo.
我们对四种不同的小鼠黑色素瘤细胞系B16-F0、-F1、-F10和-BL6的转移能力及致死率进行了表征。B16-F0是亲本细胞系。B16-F1是通过一次性选择程序获得的，B16-F10是使用菲德勒方法通过十次选择程序获得的。源自B16-F10的B16-BL6具有比B16-F10更强的侵袭活性。为了研究B16-F0、-F1、-F10和-BL6之间致死性恶性程度的差异，我们检测了经静脉接种这些细胞的同基因C57BL/6Cr小鼠的存活时间。作为对照，我们使用了C57BL/6J胚胎小鼠成纤维样半正常细胞系。小鼠形成肺转移瘤结节的能力按以下顺序逐渐增强：B16-F0、-F1和-F10（=-BL6）。接种C57BL/6J胚胎细胞（1×10⁵/只小鼠）的小鼠存活超过46天。接种B16-F0、-F1、-F10和-BL6（1×10⁵/只小鼠）的小鼠分别存活31.4±4.4（7）、25.7±2.8（7）、23.6±1.5（7）和25.3±2.3（7）天[平均值±标准差（小鼠数量）]。根据曼-惠特尼检验，接种B16-F0的组与接种-F1的组（P<0.05）、接种-F0的组与接种-BL6的组（P<0.05）以及接种-F0的组与接种-F10的组（P<0.01）有显著差异，但B16-F1组与-F10组、-F1组与-BL6组以及-F10组与-BL6组无显著差异。这些结果表明，致死性恶性程度不一定与肺定植潜力相关，甚至仅经过一次性选择的B16-F0小鼠黑色素瘤细胞也可作为体内实验性转移模型。
