Rurek M, Szklarczyk M, Adamczyk N, Michalik B, Augustyniak H
Department of Plant Molecular Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland.
Acta Biochim Pol. 2001;48(3):711-7.
A high level of the nucleotide sequence conservation was found for mitochondrial nad3 gene of carrot. Three silent nucleotide substitutions differentiate nad3 open reading frames from cytoplasmic male sterile and male fertile carrots. All these differences are preserved on the RNA level. Partial and silent editing also distinguished both carrots. Three of the C to U conversions were specific to the fertile line. In the two examined carrot lines editing did not affect the mode of alteration of encoded amino acids.
在胡萝卜线粒体nad3基因中发现了高度的核苷酸序列保守性。三个沉默核苷酸取代区分了细胞质雄性不育和雄性可育胡萝卜的nad3开放阅读框。所有这些差异在RNA水平上得以保留。部分编辑和沉默编辑也区分了这两种胡萝卜。三个C到U的转换是可育系特有的。在所检测的两个胡萝卜品系中,编辑不影响编码氨基酸的改变方式。