兔后囊膜混浊的发展

Development of posterior capsule opacification in the rabbit.

作者信息

Wallentin N, Lundgren B, Holmén J B, Lundberg C

机构信息

Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden.

出版信息

Ophthalmic Res. 2002 Jan-Feb;34(1):14-22. doi: 10.1159/000048320.

DOI:10.1159/000048320

PMID:11834880

Abstract

PURPOSE

The aim of the present study was to characterize the development of after-cataract in the rabbit by measuring its wet weight, protein, DNA and glycosaminoglycan (GAG) contents and using Scheimpflug and slitlamp analysis. Further, aqueous humor (AqH) leukocytes, protein and lens epithelial cell proliferation activity were determined.

METHODS

AqH was collected and capsular bags including after-cataract were dissected free on days 0, 1, 7, 14, 28 and 56 after cataract surgery. The wet weights were determined and the contents of DNA, protein and GAG in the capsular bags including after-cataract were analyzed. AqH was analyzed for leukocytes, protein and proliferative activity. In another set of experiments, rabbit eyes were analyzed by the Scheimpflug technique and slitlamp examination on days 0, 1, 7, 14, 28 and 56 after cataract surgery. The wet weight of the capsular bag with the after-cataract was also determined.

RESULTS

An increase was found in the wet weight (480%) and the contents of protein (221%), DNA (945%) and GAG (336%) of the capsular bags including after-cataract during the experimental period. In the AqH, all 3 variables measured, leukocytes, protein and proliferative activity, reached their highest levels on day 1 after surgery. In the second set of experiments, the wet weight of the capsular bag including after-cataract increased by 391% during the 56-day experimental period. Posterior capsule opacification (PCO), as measured by Scheimpflug analysis, increased from 0.8 to 81.7% and the scores of Elschnig's pearls as well as fibrosis, analyzed by slitlamp, increased from 0.0 to 2.8 and 3.0, respectively.

CONCLUSIONS

This study shows that the same components that are reported to be important in human PCO are also components of PCO in the rabbit. Thus, the rabbit model seems to accurately reflect human PCO development, and because PCO develops much faster in rabbits that would make the rabbit model suitable for studies to elucidate human PCO development.

摘要

目的

本研究的目的是通过测量兔后发性白内障的湿重、蛋白质、DNA和糖胺聚糖（GAG）含量，并使用Scheimpflug和裂隙灯分析来描述其发展过程。此外，还测定了房水（AqH）中的白细胞、蛋白质和晶状体上皮细胞增殖活性。

方法

在白内障手术后第0、1、7、14、28和56天收集房水，并将包含后发性白内障的囊袋游离解剖。测定湿重，并分析包含后发性白内障的囊袋中的DNA、蛋白质和GAG含量。分析房水中的白细胞、蛋白质和增殖活性。在另一组实验中，在白内障手术后第0、1、7、14、28和56天通过Scheimpflug技术和裂隙灯检查对兔眼进行分析。还测定了带有后发性白内障的囊袋的湿重。

结果

在实验期间，包含后发性白内障的囊袋的湿重（增加480%）、蛋白质含量（增加221%）、DNA含量（增加945%）和GAG含量（增加336%）均有所增加。在房水中，所测量的所有三个变量，即白细胞、蛋白质和增殖活性，在手术后第1天达到最高水平。在第二组实验中，在56天的实验期内，包含后发性白内障的囊袋的湿重增加了391%。通过Scheimpflug分析测量的后囊膜混浊（PCO）从0.8%增加到81.7%，通过裂隙灯分析的Elschnig珠和纤维化的评分分别从0.0增加到2.8和3.0。