Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, WI 53792-3220, USA.
J Ocul Pharmacol Ther. 2010 Dec;26(6):533-9. doi: 10.1089/jop.2010.0074. Epub 2010 Oct 28.
This study is aimed to determine if the serine-threonine kinase inhibitor H-7 inhibits secondary cataract after phacoemulsification in the live rabbit eye.
Eighteen rabbits underwent extracapsular lens extraction by phacoemulsification in 1 eye. The eye was treated with intravitreal H-7 (300 or 1,200 μM; n = 6 or 5) or balanced salt solution (BSS) (n = 7) immediately after the surgery and twice weekly for 10 weeks. Each eye received slit lamp biomicroscopy once a week, during which posterior capsule opacification (PCO) was evaluated. The eye was then enucleated and the lens capsule was prepared, fixed, and imaged. PCO was evaluated again on the isolated lens capsule under a phase microscope. Soemmering's ring area (SRA) and the entire lens capsule area were measured from capsule images on a computer and the percentage of SRA (PSRA) in the entire capsule area was calculated. Wet weight of the capsule (WW) was determined on a balance.
No significant difference in PCO was observed in any comparison. No significant differences in SRA, PSRA, and WW were observed between the 300 μM H-7-treated eye and the BSS-treated eye. However, SRA, PSRA, and WW in the 1,200 μM H-7-treated eye were significantly smaller than those in the BSS-treated eye [28.3 ± 16.2 vs. 61.4 ± 8.86 mm(2) (P = 0.001), 33% ± 20% vs. 65% ± 15% (P = 0.01), and 65.6 ± 27.9 vs. 127.0 ±37.3 mg (P = 0.01)].
Intravitreal H-7 (1,200 μM) significantly inhibits Soemmering's ring formation in the live rabbit eye, suggesting that agents that inhibit the actomyosin system in cells may prevent secondary cataract after phacoemulsification.
本研究旨在确定丝氨酸-苏氨酸激酶抑制剂 H-7 是否能抑制活体兔眼白内障超声乳化术后的后发性白内障。
18 只兔子在 1 只眼行白内障超声乳化摘除术。术后立即玻璃体腔内注射 H-7（300 或 1200μM；n=6 或 5）或平衡盐溶液（BSS）（n=7），术后 10 周内每周注射 2 次。每周对每只眼进行 1 次裂隙灯生物显微镜检查，评估后囊混浊（PCO）。然后眼球摘出，晶状体囊准备、固定和成像。在相差显微镜下再次评估分离晶状体囊下的 PCO。在计算机上从胶囊图像测量 Soemmering 环区（SRA）和整个晶状体囊区，计算整个囊区的 SRA 百分比（PSRA）。在天平上测定囊的湿重（WW）。
任何比较均未观察到 PCO 有显著差异。300μM H-7 治疗眼与 BSS 治疗眼的 SRA、PSRA 和 WW 无显著差异。然而，1200μM H-7 治疗眼的 SRA、PSRA 和 WW 明显小于 BSS 治疗眼[28.3±16.2 与 61.4±8.86mm(2)（P=0.001），33%±20%与 65%±15%（P=0.01），65.6±27.9 与 127.0±37.3mg（P=0.01）]。
玻璃体腔内 H-7（1200μM）显著抑制活体兔眼 Soemmering 环形成，提示抑制细胞中肌动球蛋白系统的药物可能预防白内障超声乳化术后的后发性白内障。
