Beck W W, Silverstein I
Fertil Steril. 1975 Sep;26(9):863-7.
Semen from a random group of potentially fertile men and a small number of donors who had established an artificial insemination pregnancy was mixed with a cryoprotective agent and frozen by two different methods. After 1 week under liquid nitrogen (-196 degrees C), all samples were thawed and their motilities determined. The post-thaw motilities were compared with the prefreeze motilities. The mean motility recovery rate in the group of semen samples with high counts was better than that in the group with counts in the low-normal range. The recovery rate with the slow-freeze method was slightly higher than that with the quick-freeze technique. However, the wide range of post-thaw motility among the random donors (0 to 60%) and the donors of proven fertility (15 to 45%) demonstrated that prefreeze motility and/or paternity history was no guarantee of good motility recovery following freezing. At a time when semen banking is being contemplated for "fertility insurance" prior to vasectomy, it is apparent that a trial freeze is necessary before recommending freeze-preservation of his semen to the man contemplating permanent surgical sterilization.
从一组随机抽取的可能具有生育能力的男性以及少数通过人工授精成功怀孕的捐赠者身上获取的精液，与一种冷冻保护剂混合，并通过两种不同方法进行冷冻。在液氮（-196摄氏度）中保存1周后，所有样本被解冻并测定其活力。将解冻后的活力与冷冻前的活力进行比较。高精子计数组精液样本的平均活力恢复率优于精子计数处于低正常范围组。慢冻法的恢复率略高于速冻技术。然而，随机捐赠者（0%至60%）和已证实具有生育能力的捐赠者（15%至45%）解冻后活力的广泛差异表明，冷冻前的活力和/或生育史并不能保证冷冻后有良好的活力恢复。在考虑为输精管结扎术前的“生育保险”进行精液储存时，显然在向考虑永久性手术绝育的男性推荐冷冻保存其精液之前，有必要进行一次试冻。
