Friedman S, Broder S
Fertil Steril. 1981 Mar;35(3):321-4.
Although there are sporadic reports of pregnancies using semen after long-term cryopreservation, there are no data on the success rate of this procedure and its clinical feasibility. Between 1969 and 1980, 475 men deposited semen specimens prior to vasectomy or therapeutic sterilization. Prefreeze sperm counts and motility were significantly higher in the prevasectomy group. Later, 5 wives of 177 vasectomized men and 16 wives of 183 therapeutically sterilized men returned for artificial insemination with their husbands' semen. Only 1 conception resulted among the 21 women. This occurred after a single insemination of a 7-year-old specimen obtained prevasectomy. The post-thaw motility of this specimen (45%) was better than that of most other thawed specimens. Post-thaw motilities were unpredictable despite comparable prefreeze motilities. Because of this unpredictability, semen cryopreservation is clearly no guarantee of "fertility insurance".
尽管有关于长期冷冻保存后使用精液妊娠的零星报道，但尚无该方法成功率及其临床可行性的数据。1969年至1980年间，475名男性在输精管结扎或治疗性绝育前留存了精液样本。输精管结扎术前组的冷冻前精子计数和活力明显更高。后来，177名输精管结扎男性的5名妻子以及183名治疗性绝育男性的16名妻子回来接受了用其丈夫精液进行的人工授精。21名女性中仅1例受孕。这发生在对1份输精管结扎术前获取的7年样本进行单次授精之后。该样本的解冻后活力（45%）优于大多数其他解冻样本。尽管冷冻前活力相当，但解冻后活力却无法预测。由于这种不可预测性，精液冷冻保存显然不能保证“生育保险”。
