Saitoh Tetsuroh, Mine Tetsuya, Katoh Masaru
Genetics and Cell Biology Section, Genetics Division, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
Int J Mol Med. 2002 Mar;9(3):251-6.
GIPC1/GIPC interacts with GTPase-activating protein RGS-GAIP, transmembrane protein M-SemF, receptor tyrosine kinase TrkA, integrin alpha 6A subunit, and TGF beta type III receptor. Kermit, a Xenopus orthologue of human GIPC1, interacts with Frizzled-3 (FZD3) class of WNT receptor. We have recently cloned and characterized human GIPC2 and GIPC3. Here, we identified mouse Gipc3 gene fragments by using bioinformatics, and isolated mouse Gipc3 cDNAs by using cDNA-PCR. Mouse Gipc3 gene encoded a 297-amino-acid protein, showing 86.2% total-amino-acid identity with human GIPC3. In addition to the central PDZ domain, GIPC homologous domain 1 (GH1 domain) and GH2 domain were found to be conserved among mouse Gipc3, Gipc1, Gipc2, and Xenopus Kermit. Mouse Gipc3 gene was found to consist of 6 exons, and exon-intron structure was well conserved between mouse Gipc3 gene and human GIPC3 gene. Mouse Gipc3 mRNA was relatively highly expressed in adult lung, and was also expressed in brain and testis, but was almost undetectable in 7-, 11-, 15, and 17-day whole embryos. This is the first report on molecular cloning and initial characterization of mouse Gipc3.
GIPC1/GIPC与GTP酶激活蛋白RGS - GAIP、跨膜蛋白M - SemF、受体酪氨酸激酶TrkA、整合素α6A亚基以及转化生长因子βⅢ型受体相互作用。Kermit是人类GIPC1在非洲爪蟾中的同源物，它与WNT受体的卷曲蛋白-3（FZD3）家族相互作用。我们最近克隆并鉴定了人类GIPC2和GIPC3。在此，我们利用生物信息学鉴定了小鼠Gipc3基因片段，并通过cDNA - PCR分离得到了小鼠Gipc3 cDNA。小鼠Gipc3基因编码一种297个氨基酸的蛋白质，与人类GIPC3的总氨基酸同一性为86.2%。除了中央的PDZ结构域外，还发现GIPC同源结构域1（GH1结构域）和GH2结构域在小鼠Gipc3、Gipc1、Gipc2以及非洲爪蟾Kermit中是保守的。发现小鼠Gipc3基因由6个外显子组成，并且小鼠Gipc3基因与人GIPC3基因之间的外显子-内含子结构保守性良好。小鼠Gipc3 mRNA在成年肺中相对高表达，在脑和睾丸中也有表达，但在7天、11天、15天和17天的全胚胎中几乎检测不到。这是关于小鼠Gipc3分子克隆和初步鉴定的首次报道。
