Dai C, Wang W, Cao Y
Department of Plastic & Reconstructive Surgery of Shanghai Ninth People's Hospital, Tissue Engineering Center of Shanghai Second Medical University, Shanghai 200011, China.
Zhonghua Zheng Xing Wai Ke Za Zhi. 2001 Nov;17(6):366-8.
To develop an effective new material for repair of nerve defects.
The 4-week pre-degenerative and normal sciatic nerve segments of SD rats were extracted with the detergent lysophosphatidylcholine, which resulted in acellular nerve basal lamina tubes, called EDNG (extracted degenerative nerve grafts) and ENG (extracted normal nerve grafts) respectively. The morphology of the acellular nerve basal lamina tubes was revealed by conventional histological examinations, transmission electron microscopy and immunohistochemistry method. The basal lamina tubes, as allografts, were used to repair a 15 mm-gap of sciatic nerve in Wistar rats. In the control group nerve autografts (NAG) was applied. Evaluation included general observation, electromyographic examination, muscle measurement and histological observation of serial sections at 12 weeks after surgery.
The EDNG had more ideal frame, better resilience than ENG. There was no any cell debris in EDNG, whereas in the inner wall of the tubes immunohistochemical analysis revealed clear positive staining and strong immunoreactivity of laminin under immunoelectron microscopy. After peripheral nerves were repaired, all the animals of the three groups had restoration of function to a certain extent. The result of the NAG group was the best, the EDNG group was the next and the ENG group was the inferior.
This new material offers a possible solution to repair of a short defect of a peripheral nerve.
研发一种用于修复神经缺损的有效新材料。
用去污剂溶血磷脂酰胆碱提取4周龄SD大鼠的预变性和正常坐骨神经段，得到无细胞神经基膜管，分别称为EDNG（提取的变性神经移植物）和ENG（提取的正常神经移植物）。通过常规组织学检查、透射电子显微镜和免疫组织化学方法揭示无细胞神经基膜管的形态。将基膜管作为同种异体移植物用于修复Wistar大鼠坐骨神经15毫米的缺损。对照组应用神经自体移植物（NAG）。评估包括术后12周的一般观察、肌电图检查、肌肉测量和连续切片的组织学观察。
EDNG比ENG具有更理想的结构和更好的弹性。EDNG中没有任何细胞碎片，而在管的内壁免疫组织化学分析显示在免疫电子显微镜下层粘连蛋白呈明显阳性染色和强免疫反应性。周围神经修复后，三组所有动物的功能均有一定程度的恢复。NAG组结果最佳，EDNG组次之，ENG组最差。
这种新材料为修复周围神经的短缺损提供了一种可能的解决方案。
