Takeuchi S, Azuma R, Nakajima Y, Suto T
Natl Inst Anim Health Q (Tokyo). 1979 Fall;19(3):77-82.
A protease antigen was prepared from the culture supernatant of Corynebacterium pyogenes by concentrating with a flash evaporator and ultrafiltration. It was adjusted to the concentration of 32 units by the single radial immunodiffusion with a tentative standard serum. In the immunodiffusion test, the antigen of 4 units reacted enough with sera having an antibody titer ranging from 1 to 128. As a result, it was decided that the antigen of 4 units should be used in the immunodiffusion test for the detection of protease antibody. By the immunodiffusion test, protease antibody was demonstrated in about 35% of 443 sera from pigs collected at random. The antibody titer showed the distribution of 2 peaks. The summits of the two peaks were seen at 4 and 32 of antibody titer, respectively. The valley between the two peaks was seen at 16 of titer. From the result, a diagnostic criterion of the immunodiffusion test was decided provisionally as follows: above 16 of antibody titer is positive, 1 to 8 suspect, and less than 1 negative. On the other hand, protease antibody was demonstrated in sera from 13 of 14 pigs carrying abscesses from which C. pyogenes had been isolated. Its titer was 8 (in 2 pigs), 16 (in 1), 32 (in 3), 64 (in 6), and 128 (in 1). From these results, it was proposed that the immunodiffusion test with protease antigen be used for the diagnosis of C. pyogenes infection in pigs.
用闪蒸蒸发器浓缩和超滤法从化脓棒状杆菌培养上清液中制备蛋白酶抗原。用暂定标准血清通过单向放射免疫扩散法将其调整至32单位浓度。在免疫扩散试验中，4单位的抗原与抗体效价为1至128的血清有足够反应。结果确定，在检测蛋白酶抗体的免疫扩散试验中应使用4单位的抗原。通过免疫扩散试验，在随机采集的443份猪血清中约35%检测到蛋白酶抗体。抗体效价呈双峰分布。两个峰的峰值分别出现在抗体效价为4和32处。两个峰之间的谷值出现在效价为16处。根据该结果，免疫扩散试验的诊断标准暂定为：抗体效价高于16为阳性，1至8为可疑，低于1为阴性。另一方面，从14头分离出化脓棒状杆菌的患脓肿猪的血清中，有13份检测到蛋白酶抗体。其效价分别为8（2头猪）、16（1头）、32（3头）、64（6头）和128（1头）。根据这些结果，建议用蛋白酶抗原免疫扩散试验诊断猪化脓棒状杆菌感染。
