Jennes Wim, Sawadogo Souleymane, Koblavi-Dème Stéphania, Vuylsteke Bea, Maurice Chantal, Roels Thierry H, Chorba Terence, Nkengasong John N, Kestens Luc
Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium.
AIDS Res Hum Retroviruses. 2002 Feb 10;18(3):171-7. doi: 10.1089/08892220252781220.
The role of beta-chemokines in controlling HIV replication in vivo is still controversial. Therefore, the association between HIV-1 plasma viral load and the capacity of CD4(+) and CD8(+) T cells to produce beta-chemokines was studied in 28 antiretroviral drug-naïve HIV-1-infected female sex workers in Abidjan, Côte d'Ivoire. Percentages of beta-chemokine-positive T cells were measured in peripheral blood mononuclear cells by flow cytometry after intracellular staining for RANTES (regulated on activation, normal T expressed and secreted), macrophage inflammatory protein (MIP)-1alpha, and MIP-1beta. HIV-1-infected subjects had higher percentages of MIP-1alpha- and MIP-1beta-positive CD4(+) and CD8(+) T cells (p < 0.02) and of RANTES-positive CD8(+) T cells (p = 0.054) than uninfected controls. Percentages of RANTES- and MIP-1beta-positive CD8(+) T cells correlated directly with HIV-1 plasma viral load (p < 0.02). Percentages of beta-chemokine-positive CD4(+) and CD8(+) T cells correlated directly with percentages of HLA-DR-positive T cells (p < 0.02) and inversely (except RANTES in CD4(+) T cells) with absolute numbers of CD4(+) T cells (p < 0.05) in peripheral blood. These data indicate that increased percentages of beta-chemokine-producing T cells in HIV-1-infected subjects correlate with disease progression and are a sign of viremia-driven chronic T cell activation.
β趋化因子在体内控制HIV复制中的作用仍存在争议。因此，在科特迪瓦阿比让的28名未接受过抗逆转录病毒药物治疗的HIV-1感染女性性工作者中，研究了HIV-1血浆病毒载量与CD4(+)和CD8(+) T细胞产生β趋化因子能力之间的关联。在对调节激活正常T细胞表达和分泌因子（RANTES）、巨噬细胞炎性蛋白（MIP）-1α和MIP-1β进行细胞内染色后，通过流式细胞术检测外周血单个核细胞中β趋化因子阳性T细胞的百分比。与未感染的对照组相比，HIV-1感染受试者中MIP-1α和MIP-1β阳性的CD4(+)和CD8(+) T细胞百分比更高（p < 0.02），RANTES阳性的CD8(+) T细胞百分比更高（p = 0.054）。RANTES和MIP-1β阳性的CD8(+) T细胞百分比与HIV-1血浆病毒载量直接相关（p < 0.02）。β趋化因子阳性的CD4(+)和CD8(+) T细胞百分比与HLA-DR阳性T细胞百分比直接相关（p < 0.02），与外周血中CD4(+) T细胞的绝对数量呈负相关（CD4(+) T细胞中的RANTES除外）（p < 0.05）。这些数据表明，HIV-1感染受试者中产生β趋化因子的T细胞百分比增加与疾病进展相关，是病毒血症驱动的慢性T细胞激活的标志。
