一种用于核酸中明确核磁共振光谱归属的位点特异性低丰度（15）N、（13）C同位素标记方法。
A site-specific low-enrichment (15)N,(13)C isotope-labeling approach to unambiguous NMR spectral assignments in nucleic acids.
作者信息
Phan Anh Tuân, Patel Dinshaw J
机构信息
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center New York, New York 10021, USA.
出版信息
J Am Chem Soc. 2002 Feb 20;124(7):1160-1. doi: 10.1021/ja011977m.
PMID:11841271
Abstract
We demonstrate that base and sugar protons within a DNA oligomer can be assigned unambiguously following site-specific 15N,13C isotope incorporation at levels as low as 1% enrichment. This simple and cost-effective methodology is demonstrated on the d(GGGTTCAGG) DNA sequence, which forms a dimeric G-quadruplex containing G.G.G.G tetrads sandwiched between G.(C-A) triads.
摘要
我们证明，在低至1%丰度的位点特异性15N、13C同位素掺入后，DNA寡聚物中的碱基和糖质子可以明确地进行归属。这种简单且经济高效的方法在d(GGGTTCAGG) DNA序列上得到了验证，该序列形成了一个二聚体G-四链体，其中包含夹在G.(C-A)三联体之间的G.G.G.G四联体。
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