School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Singapore.
Laboratoire de Biologie Structurale de la Cellule (BIOC), Ecole Polytechnique, CNRS-UMR7654, Institut Polytechnique de Paris, Palaiseau 91128, France.
Nucleic Acids Res. 2021 Feb 22;49(3):1724-1736. doi: 10.1093/nar/gkaa1259.
G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures.
G-四链体（G4）DNA 结构具有左手骨架进展，具有独特且保守的结构特征。对结构的序列依赖性研究揭示了形成左手 G4 的先决条件和一些最小基序。为了扩展边界，我们探索了左手 G4 对凸起存在的适应性。在这里，我们展示了两个容纳一个、两个和三个凸起的左手 G4 的 X 射线晶体结构和一个 NMR 溶液结构。与右手 G4 相比，左手 G4 中的凸起具有明显的特征。阐明复杂的结构细节将有助于理解这些独特结构的可能作用和局限性。
