利用简单序列重复区间（ISSR）扩增对大麻进行DNA指纹图谱分析。
DNA fingerprinting of Cannabis sativa using inter-simple sequence repeat (ISSR) amplification.
作者信息
Kojoma Mareshige, Iida Osamu, Makino Yukiko, Sekita Setsuko, Satake Motoyoshi
机构信息
Izu Experimental Station for Medicinal Plants, National Institute of Health Sciences, Japan.
出版信息
Planta Med. 2002 Jan;68(1):60-3. doi: 10.1055/s-2002-19875.
Chemical analysis of cannabinoid, and Inter-Simple Sequence Repeat (ISSR) fingerprinting of DNA were used to identify different samples of Cannabis sativa L. for forensic purposes. Three samples were classified into two types, tetrahydrocannabinol (THC) and cannabidiol (CBD) chemo-types, by high performance liquid chromatography (HPLC). The two samples of the CBD type were not distinguished by their HPLC patterns. ISSR fingerprinting identified polymorphic DNA patterns between these samples. ISSR fingerprinting clearly differentiated between cannabis samples that could not be achieved by HPLC analysis.
为了法医鉴定目的，采用大麻素的化学分析以及DNA的简单序列重复区间（ISSR）指纹图谱技术来识别不同的大麻样品。通过高效液相色谱法（HPLC）将三个样品分为两种类型，即四氢大麻酚（THC）化学类型和大麻二酚（CBD）化学类型。CBD类型的两个样品在HPLC图谱上无法区分。ISSR指纹图谱识别出了这些样品之间的多态性DNA模式。ISSR指纹图谱能够清晰地区分HPLC分析无法区分的大麻样品。
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