从妊娠早期蜕膜中克隆恒河猴杀伤细胞免疫球蛋白样受体（KIRs）。

Cloning of rhesus monkey killer-cell Ig-like receptors (KIRs) from early pregnancy decidua.

作者信息

Grendell R L, Hughes A L, Golos T G

机构信息

Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, Wisconsin 53715-1299, USA.

出版信息

Tissue Antigens. 2001 Nov;58(5):329-34. doi: 10.1034/j.1399-0039.2001.580507.x.

DOI:10.1034/j.1399-0039.2001.580507.x

PMID:11844144

Abstract

To define the killer-cell Ig-like receptors (KIR) molecules expressed within the decidua in the rhesus monkey, an early pregnancy rhesus decidual library was screened with an oligonucleotide located within the KIR D2 domain. We obtained 26 full-length clones which represented 11 mRNAs. The cDNAs encode proteins containing 2 or 3 Ig domains, and short or long cytoplasmic domains. The long domains contained immunotyrosine inhibitory motifs (ITIM) motifs indicating inhibitory function. Three clones contained single or dinucleotide deletions within the D1 domain, which would result in proteins truncated within this domain. Phylogenetic analysis of the Ig domains supports a D0 - D2 organization of rhesus KIR2DL4.1, similar to human KIR2DL4 and KIR2DL5. The rhesus KIR2DL4.1 transcript contains a long cytoplasmic tail homologous with human KIR2DL4.

摘要

为了确定恒河猴蜕膜中表达的杀伤细胞免疫球蛋白样受体（KIR）分子，用位于KIR D2结构域内的寡核苷酸筛选了一个早期妊娠恒河猴蜕膜文库。我们获得了26个全长克隆，代表11种mRNA。这些cDNA编码的蛋白质含有2个或3个免疫球蛋白结构域，以及短或长的细胞质结构域。长结构域包含免疫酪氨酸抑制基序（ITIM），表明具有抑制功能。三个克隆在D1结构域内含有单核苷酸或二核苷酸缺失，这将导致该结构域内的蛋白质截短。对免疫球蛋白结构域的系统发育分析支持恒河猴KIR2DL4.1的D0 - D2组织形式，类似于人类KIR2DL4和KIR2DL5。恒河猴KIR2DL4.1转录本包含与人KIR2DL4同源的长细胞质尾巴。