Al-Zuhair A, Gosling J A, Dixon J S
J Anat. 1975 Sep;120(Pt 1):81-93.
A combination of light microscopic, neurohistochemical and electron microscopic techniques were used to study the distribution of autonomic nerves in the seminal vesicle, and in the submucosa of the ductus deferens, in the guinea-pig. In routine histological preparations, the muscle coat of the seminal vesicle consisted largely of circularly orientated smooth muscle cells with an additional longitudinal layer confined to its urethral end. Using fluorescence microscopy, a rich plexus of noradrenergic nerves was demonstrated throughout the muscle coat of the seminal vesicle and this was confirmed electron microscopically. Numerous noradrenergic terminal regions were frequently observed 10-100 nm from adjacent smooth muscle cells, and catecholamine-containing nerve cell bodies were observed in the adventitia of the urethral end of the seminal vesicle. With the light microscope, a rich plexus of AChE+ve nerves was found in the circular muscle layer but the outer longitudinal layer contained few similar nerves. AChE+ve ganglion cells were confined to the adventitia of the urethral end of the seminal vesicle. Electron microscopy revealed a second type of terminal region containing small agranular vesicles, the distribution of which corresponded to that of the AChE+ve nerves observed by light microscopy. In both layers, these terminal regions were associated with other axons, although the circular muscle layer also contained single axons of this type in close association with adjacent smooth muscle cells. Very few noradrenergic nerves were found in the submucosa of the seminal vesicle and ductus deferens and those which were observed usually accompanied the vascular supply. In contrast, a rich AChE+ve nerve network was observed in the submucosa of both the seminal vesicle and the ductus deferens. Electron microscopy revealed numerous groups of submucosal axons, many of which appeared varicose and contained small, agranular vesicles. Similar intra-epithelial axons were occasionally observed in both organs. These findings have been discussed in relation to the possible functional significance of this extensive autonomic innervation.
运用光学显微镜、神经组织化学和电子显微镜技术相结合的方法，对豚鼠精囊以及输精管黏膜下层的自主神经分布进行了研究。在常规组织学切片中，精囊的肌层主要由呈环形排列的平滑肌细胞组成，在尿道端还有一层额外的纵行肌层。利用荧光显微镜观察发现，精囊肌层中存在丰富的去甲肾上腺素能神经丛，这一点通过电子显微镜得到了证实。在距相邻平滑肌细胞10 - 100纳米处经常能观察到大量去甲肾上腺素能终末区域，并且在精囊尿道端的外膜中发现了含儿茶酚胺的神经细胞体。通过光学显微镜观察发现，环形肌层中有丰富的乙酰胆碱酯酶阳性神经丛，但外层纵行肌层中此类神经较少。乙酰胆碱酯酶阳性神经节细胞局限于精囊尿道端的外膜。电子显微镜显示存在第二种终末区域，其中含有小的无颗粒小泡，其分布与光学显微镜下观察到的乙酰胆碱酯酶阳性神经的分布相对应。在这两层中，这些终末区域都与其他轴突相关联，尽管环形肌层中也有这种类型的单个轴突与相邻平滑肌细胞紧密相连。在精囊和输精管的黏膜下层中发现的去甲肾上腺素能神经极少，所观察到的那些神经通常伴随血管分布。相比之下，在精囊和输精管的黏膜下层均观察到丰富的乙酰胆碱酯酶阳性神经网络。电子显微镜显示黏膜下有许多轴突束，其中许多看起来呈曲张状并含有小的无颗粒小泡。在这两个器官中偶尔也能观察到类似的上皮内轴突。结合这种广泛的自主神经支配可能具有的功能意义，对这些发现进行了讨论。
