黑腹果蝇的小环状DNA：染色体同源性和动力学复杂性。
Small circular DNA of Drosophila melanogaster: chromosomal homology and kinetic complexity.
作者信息
Stanfield S W, Lengyel J A
出版信息
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6142-6. doi: 10.1073/pnas.76.12.6142.
Abstract
Nucleic acid reassociation techniques were used to determine the kinetic complexity of small circular DNA in cultured cells of Drosophila melanogaster. Two kinetic components are present. One of these constitutes 82% of the mass of the circular DNA and has a complexity of 1.8 x 10(4) nucleotide pairs; the other constitutes 18% of the mass and the a significantly higher but undefined sequence complexity. We have demonstrated that these circular molecules hybridize to middle repetitive chromosomal sequences by hybridization of in vitro-labeled circular DNA tracer with a vast excess of Drosophila chromosomal DNA. Thermal stability measurements indicate that base-pair mismatch between small circular DNA and middle repetitive chromosomal DNA does not exceed 2%. We discuss possible functions of these small circular DNAs in light of the above findings.
摘要
核酸复性技术被用于测定黑腹果蝇培养细胞中小环状DNA的动力学复杂度。存在两个动力学组分。其中一个占环状DNA质量的82%，复杂度为1.8×10⁴个核苷酸对；另一个占质量的18%，序列复杂度明显更高但未明确界定。我们通过体外标记的环状DNA示踪剂与大量果蝇染色体DNA杂交，证明了这些环状分子与中度重复染色体序列杂交。热稳定性测量表明，小环状DNA与中度重复染色体DNA之间的碱基对错配不超过2%。根据上述发现，我们讨论了这些小环状DNA可能的功能。
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