Pont G, Degroote F, Picard G
Laboratoire de Génétique, UA 360 CNRS, Université Blaise Pascal-Clermont-Fd. II, Aubière, France.
Nucleic Acids Res. 1988 Sep 26;16(18):8817-33. doi: 10.1093/nar/16.18.8817.
From extrachromosomal covalently closed circular DNA molecules purified from Drosophila melanogaster embryos, we have isolated 24 clones homologous to the histone tandemly repeated gene family. Some of the clones harbor one of the two main types of genomic repeated units of 4.8 and 5.0 kb. and probably result from homologous recombination. The remaining clones have a size ranging from 0.2 to 2.5 kb. and most of them carry a single fragment of the repeated unit. Nucleotide sequences of the junction region of six of these clones indicate they are generated by illegitimate recombination between short (8-15 bp.) imperfect direct repeats. The data suggest that most of the histone homologous circular DNA molecules are deleted histone units.
从黑腹果蝇胚胎中纯化得到的染色体外共价闭合环状DNA分子中，我们分离出了24个与组蛋白串联重复基因家族同源的克隆。其中一些克隆含有4.8和5.0 kb这两种主要基因组重复单元中的一种，可能是同源重组的结果。其余克隆的大小在0.2到2.5 kb之间，它们中的大多数携带重复单元的单个片段。其中六个克隆的连接区域的核苷酸序列表明，它们是由短（8 - 15 bp）的不完全直接重复序列之间的异常重组产生的。数据表明，大多数组蛋白同源环状DNA分子是缺失的组蛋白单元。
