利用神户大学资源家系构建的鸡连锁图谱对肌营养不良症AM位点进行定位。

Localization of the muscular dystrophy AM locus using a chicken linkage map constructed with the Kobe University resource family.

作者信息

Lee E J, Yoshizawa K, Mannen H, Kikuchi H, Kikuchi T, Mizutani M, Tsuji S

机构信息

Laboratory of Animal Breeding and Genetics, Faculty of Agriculture, Kobe University, Kobe, 657-8501, Japan.

出版信息

Anim Genet. 2002 Feb;33(1):42-8. doi: 10.1046/j.1365-2052.2002.00825.x.

DOI:10.1046/j.1365-2052.2002.00825.x

PMID:11849136

Abstract

A chicken linkage map, constructed with the Kobe University (KU) resource family, was used to locate the genetic locus for muscular dystrophy of abnormal muscle type (AM). The KU resource family is a backcross pedigree with 55 offspring produced from the mating of a White Leghorn F-line (WL-F) male and a hybrid female produced from a cross between the WL-F male and a female of the Fayoumi OPN line who was homozygous for the AM gene. In total, 872 loci were genotyped on the pedigree; 749 (86%) were informative and mapped to 38 linkage groups. These informative loci included 649 AFLPs, 93 MS, three functional genes, the AM locus, sex phenotype, and two red blood cell loci. The remaining 123 markers were unlinked. Nineteen of the 38 KU linkage groups were assigned to macrochromosomes 1-8 and 11 microchromosomes including chromosome W, while 19 linkage groups were unassigned. The total map was 3569 cM in length, with an average marker interval of 4.8 cM. The AM locus was mapped 130 cM from the distal end of chromosome 2q.

摘要

利用由神户大学（KU）资源家系构建的鸡连锁图谱来定位异常肌肉类型（AM）肌营养不良的基因座。KU资源家系是一个回交谱系，由一只白来航F系（WL-F）雄性与一只由WL-F雄性与法尤米OPN系雌性杂交产生的杂种雌性交配产生了55个后代，该法尤米OPN系雌性对AM基因是纯合的。总共对该谱系中的872个基因座进行了基因分型；其中749个（86%）具有信息性，并被定位到38个连锁群中。这些具有信息性的基因座包括649个扩增片段长度多态性（AFLP）、93个微卫星（MS）、三个功能基因、AM基因座、性别表型和两个红细胞基因座。其余123个标记未连锁。38个KU连锁群中的19个被分配到1 - 8号大染色体和包括W染色体在内的11个微染色体上，而19个连锁群未被分配。总图长为3569厘摩（cM），平均标记间隔为4.8 cM。AM基因座被定位在距2号染色体长臂远端130 cM处。