Horikoshi Haruko, Kinomoto Masanobu, Kurosu Takeshi, Komoto Satoshi, Shiraga Miki, Otake Toru, Mukai Tetsu, Ikuta Kazuyoshi
Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
Virology. 2002 Feb 1;293(1):94-102. doi: 10.1006/viro.2001.1272.
A significant increase in the CD38(+) population among T lymphocytes has been observed in human immunodeficiency virus type 1 (HIV-1)-infected carriers. We previously reported a higher replication rate of T-tropic HIV-1 in the CD4(+)CD38(+)CD62L(+) than CD38(-) subset under conditions of mitogen stimulation after infection. Here, we revealed a similarly high susceptibility in the CD38(+) subset on culture with conditioned medium containing Th2 cytokine, interleukin (IL)-4 that was produced endogenously from this subset on stimulation with mitogen or anti-CD3 antibody for 3 days. The contribution of IL-4 to the upregulated production of virus in the CD38(+) subset was confirmed by culture of this subset with recombinant human IL-4. In contrast, the rate of replication in the CD38(-) subset was not augmented in the conditioned medium from either subset or with IL-4. However, there were no differences in the surface expression of IL-4 receptor or HIV-1 receptors CD4 and CXCR4 between the two subsets. Thus, the CD4(+)CD38(+)CD62L(+) subset comprises a specific cell population secreting endogenous Th2 cytokine that contributes to the efficient production of T-tropic HIV-1 through upregulation at a certain stage of the viral life cycle, probably after the adsorption step.
在人类免疫缺陷病毒1型（HIV-1）感染携带者中，已观察到T淋巴细胞中CD38(+)群体显著增加。我们之前报道，感染后在有丝分裂原刺激条件下，嗜T细胞型HIV-1在CD4(+)CD38(+)CD62L(+)亚群中的复制率高于CD38(-)亚群。在此，我们发现，用含有Th2细胞因子白细胞介素（IL）-4的条件培养基培养时，CD38(+)亚群具有同样高的易感性，IL-4是该亚群在有丝分裂原或抗CD3抗体刺激3天后内源性产生的。用重组人IL-4培养该亚群，证实了IL-4对CD38(+)亚群中病毒产生上调的作用。相比之下，CD38(-)亚群在来自任一亚群的条件培养基中或用IL-4培养时，其复制率均未增加。然而，两个亚群之间IL-4受体或HIV-1受体CD4和CXCR4的表面表达没有差异。因此，CD4(+)CD38(+)CD62L(+)亚群包含一个分泌内源性Th2细胞因子的特定细胞群体，该细胞因子通过在病毒生命周期的某个阶段（可能在吸附步骤之后）上调，有助于嗜T细胞型HIV-1的高效产生。
