Tietz H-J, Hopp M, Gräser Y
Department of Dermatology, Humboldt University, Berlin, Germany.
Mycoses. 2002 Feb;45(1-2):10-4.
Trichophyton raubitschekii is a rare dermatophyte which belongs to the Trichophyton rubrum species complex. Since 1981, only a few cases of dermatophytosis due to this anthropophilic causative agent were published. In this paper the authors report the first cases of Tinea corporis caused by Trichophyton raubitschekii (syn. T. rubrum) in Europe. The patients, one immigrant from Ghana and three from Cameroon, had typical lesions of tinea corporis. Four strains were isolated and characterized by conventional and molecular methods. On morphological and physiological grounds the isolates were identified as T. raubitschekii by the following phenotypical features: (1) velvety colony texture; (2) brown pigment; (3) abundant macroconidia and (4) positive urease activity. Molecular diagnostics were performed by single strand conformation polymorphism (SSCP) and sequence analysis of the ATPase9 intron of the mitochondrial (mt) DNA and the internal transcribed spacer (ITS) region of the rDNA, respectively. The ITS sequences and SSCP patterns of the ATPase9 intron were found to be identical among the four strains and also when compared to reference strains of T. rubrum. As shown in the present paper, T. raubitschekii is genetically identical to T. rubrum but differs in some phenotypical characteristics. Since misidentification with other dark-coloured dermatophyte variants is possible, medical mycologists should bear in mind the special morphological characteristics of T. raubitschekii (syn. T. rubrum) for future identifications.
劳氏毛癣菌是一种罕见的皮肤癣菌，属于红色毛癣菌复合种。自1981年以来，仅有少数几例由这种亲人性病原体引起的皮肤癣菌病病例被报道。在本文中，作者报告了欧洲首例由劳氏毛癣菌（同义词：红色毛癣菌）引起的体癣病例。患者中有一名来自加纳的移民以及三名来自喀麦隆的患者，他们都有典型的体癣皮损。分离出了四株菌株，并通过传统方法和分子方法进行了鉴定。基于形态学和生理学依据，通过以下表型特征将分离株鉴定为劳氏毛癣菌：（1）菌落质地天鹅绒状；（2）棕色色素；（3）大量大分生孢子；（4）尿素酶活性阳性。分别通过单链构象多态性（SSCP）以及线粒体（mt）DNA的ATPase9内含子和核糖体DNA（rDNA）的内部转录间隔区（ITS）区域的序列分析进行分子诊断。发现这四株菌株的ITS序列和ATPase9内含子的SSCP图谱彼此相同，并且与红色毛癣菌的参考菌株相比也是如此。如本文所示，劳氏毛癣菌在基因上与红色毛癣菌相同，但在一些表型特征上有所不同。由于可能与其他深色皮肤癣菌变种发生误鉴定，医学真菌学家在未来的鉴定中应牢记劳氏毛癣菌（同义词：红色毛癣菌）的特殊形态特征。
