Yokouchi Y, Shibasaki M, Noguchi E, Nakayama J, Ohtsuki T, Kamioka M, Yamakawa-Kobayashi K, Ito S, Takeda K, Ichikawa K, Nukaga Y, Matsui A, Hamaguchi H, Arinami T
Department of Medical Genetics, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan.
Genes Immun. 2002 Feb;3(1):9-13. doi: 10.1038/sj.gene.6363815.
Seasonal allergic rhinitis (SAR) is an inflammatory disease of the nose and eyes that follows sensitization to air-born pollens. We conducted a genome-wide linkage screening of 48 Japanese families (188 members) with orchard grass (OG)-sensitive SAR children (67 affected sib-pairs) in a farming community in central Japan where OG was planted for apple farming and OG pollen is a major cause of SAR. We used the GENEHUNTER program to performed nonparametric multipoint linkage analysis for OG-sensitive SAR as a qualitative trait and for log total serum IgE levels and OG-RAST IgE levels as quantitative traits. Genotyping data of 400 microsatellite markers suggested linkage of SAR to chromosomes 1p36.2, 4q13.3, and 9q34.3 (P < 0.001), linkage of serum total IgE levels to 3p24.1, 5q33.1, 12p13.1, and 12q24.2 (P < 0.001), and linkage of OG-RAST IgE levels to 4p16.1, 11q14.3, and 16p12.3 (P < 0.001). Weak evidence for linkage of SAR to 5q33.1 was also observed (P = 0.01). All these regions, with the exception of 9q34.3, have been previously reported to be linked to asthma and/or atopy. These data suggest that, although loci linked to SAR are likely to be common to asthma, a strong contribution by specific gene(s) to OG-sensitive SAR is unlikely.
季节性变应性鼻炎（SAR）是一种鼻和眼的炎症性疾病，继发于对空气传播花粉的致敏。我们在日本中部一个种植果园草（OG）用于苹果种植且OG花粉是SAR主要病因的农业社区，对48个日本家庭（188名成员）中对OG敏感的SAR儿童（67对受累同胞对）进行了全基因组连锁筛查。我们使用GENEHUNTER程序对OG敏感的SAR作为定性性状以及对总血清IgE水平和OG-RAST IgE水平作为定量性状进行非参数多点连锁分析。400个微卫星标记的基因分型数据表明，SAR与染色体1p36.2、4q13.3和9q34.3连锁（P < 0.001），血清总IgE水平与3p24.1、5q33.1、12p13.1和12q24.2连锁（P < 0.001），OG-RAST IgE水平与4p16.1、11q14.3和16p12.3连锁（P < 0.001）。还观察到SAR与5q33.1连锁的弱证据（P = 0.01）。除9q34.3外，所有这些区域先前均已报道与哮喘和/或特应性相关。这些数据表明，尽管与SAR连锁的基因座可能与哮喘共有，但特定基因对OG敏感的SAR的强烈贡献不太可能。
