粟酒裂殖酵母的基因组序列。

The genome sequence of Schizosaccharomyces pombe.

作者信息

Wood V, Gwilliam R, Rajandream M-A, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle E J, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail M A, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor R G, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Schäfer M, Müller-Auer S, Gabel C, Fuchs M, Düsterhöft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl T M, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dréano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves S J, Xiang Z, Hunt C, Moore K, Hurst S M, Lucas M, Rochet M, Gaillardin C, Tallada V A, Garzon A, Thode G, Daga R R, Cruzado L, Jimenez J, Sánchez M, del Rey F, Benito J, Domínguez A, Revuelta J L, Moreno S, Armstrong J, Forsburg S L, Cerutti L, Lowe T, McCombie W R, Paulsen I, Potashkin J, Shpakovski G V, Ussery D, Barrell B G, Nurse P

机构信息

The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.

出版信息

Nature. 2002 Feb 21;415(6874):871-80. doi: 10.1038/nature724.

DOI:10.1038/nature724

PMID:11859360

Abstract

We have sequenced and annotated the genome of fission yeast (Schizosaccharomyces pombe), which contains the smallest number of protein-coding genes yet recorded for a eukaryote: 4,824. The centromeres are between 35 and 110 kilobases (kb) and contain related repeats including a highly conserved 1.8-kb element. Regions upstream of genes are longer than in budding yeast (Saccharomyces cerevisiae), possibly reflecting more-extended control regions. Some 43% of the genes contain introns, of which there are 4,730. Fifty genes have significant similarity with human disease genes; half of these are cancer related. We identify highly conserved genes important for eukaryotic cell organization including those required for the cytoskeleton, compartmentation, cell-cycle control, proteolysis, protein phosphorylation and RNA splicing. These genes may have originated with the appearance of eukaryotic life. Few similarly conserved genes that are important for multicellular organization were identified, suggesting that the transition from prokaryotes to eukaryotes required more new genes than did the transition from unicellular to multicellular organization.

摘要

我们已对裂殖酵母（粟酒裂殖酵母）的基因组进行了测序和注释，该基因组所含蛋白质编码基因数量为真核生物中记录到的最少：4824个。着丝粒在35至110千碱基（kb）之间，包含相关重复序列，包括一个高度保守的1.8 kb元件。基因上游区域比芽殖酵母（酿酒酵母）的更长，这可能反映了更广泛的调控区域。约43%的基因含有内含子，内含子共有4730个。50个基因与人类疾病基因有显著相似性；其中一半与癌症相关。我们鉴定出了对真核细胞组织很重要的高度保守基因，包括那些对细胞骨架、区室化、细胞周期控制、蛋白水解、蛋白质磷酸化和RNA剪接所必需的基因。这些基因可能起源于真核生物生命的出现。几乎未鉴定出对多细胞组织很重要的类似保守基因，这表明从原核生物到真核生物的转变比从单细胞到多细胞组织的转变需要更多新基因。