Timerbaev Andrei R, Küng Angelika, Keppler Bernhard K
Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences, Moscow.
J Chromatogr A. 2002 Feb 1;945(1-2):25-44. doi: 10.1016/s0021-9673(01)01489-3.
A great deal of research has been carried out in recent years on developing high-efficiency capillary electrophoresis (CE) techniques that are able to separate rapidly and selectively ionic platinum metal species in a wide variety of their complexed forms. Using a range of illustrative examples, this review examines the potential and utilization of various CE separation approaches and detection modes in this expanding area. Also covered are CE procedures suitable for solving practical analytical problems and for platinum metal speciation purposes. Presenting a comprehensive treatise on the evolving practices of CE concerning platinum anticancer drugs--in particular, the examination of the stability of intact drugs, the separation and identification of products of their metabolism and interactions with biomolecules (including kinetic studies of the binding behavior)--this paper witnesses a welcome shift of the main research activities to those performed under physiological conditions.
近年来，人们开展了大量研究，致力于开发高效毛细管电泳（CE）技术，该技术能够快速、选择性地分离各种复杂形式的离子态铂金属物种。本文通过一系列示例，探讨了这一不断发展领域中各种CE分离方法和检测模式的潜力及应用。还介绍了适用于解决实际分析问题及铂金属形态分析的CE程序。本文全面论述了CE在铂类抗癌药物方面不断发展的实践——特别是对完整药物稳定性的研究、其代谢产物及与生物分子相互作用产物的分离与鉴定（包括结合行为的动力学研究），见证了主要研究活动可喜地转向在生理条件下开展的研究。
