Institute of Chemistry, University of Bialystok, Bialystok, Poland.
Anal Chim Acta. 2013 Jul 5;786:39-46. doi: 10.1016/j.aca.2013.05.031. Epub 2013 May 27.
The determination of quercetin and rutin by flow injection analysis (FIA) and capillary electrophoresis (CE) using electrochemical detection was described. These flavonoids were determined at normal (unheated) and hot platinum microelectrodes using cyclic voltammetry. When quercetin or rutin is reaching the platinum electrode, a change of the current in the region of the platinum oxide formation is observed. Integration of the current changes in this in this region creates analytical signals in the form of peaks. An increase of temperature to about 76°C in a small zone adjacent to the microelectrode causes an increase of the analytical signal by more than 6 times under FIA conditions. This method enables the use of hot microelectrodes as detectors in HPLC or CE. In CE the improvement of the analytical signal at hot microelectrodes is smaller than in FIA and increase only 1.3-3.4 times. Heated microelectrodes were used for analysis of the flavonoids in natural samples of the plant (extract of sea buckthorn) and a pharmaceutical preparation (Cerutin).
采用电化学检测的流动注射分析（FIA）和毛细管电泳（CE）法测定槲皮素和芦丁。在未加热和热铂微电极上，使用循环伏安法测定这些黄酮类化合物。当槲皮素或芦丁到达铂电极时，观察到在铂氧化物形成区域电流的变化。在该区域中对电流变化进行积分，以峰的形式产生分析信号。在紧邻微电极的小区域中，将温度升高到约 76°C，在 FIA 条件下，分析信号增加超过 6 倍。该方法允许在 HPLC 或 CE 中使用热微电极作为检测器。在 CE 中，在热微电极上分析信号的提高小于 FIA，仅增加 1.3-3.4 倍。加热的微电极用于分析植物天然样品（沙棘提取物）和药物制剂（Cerutin）中的黄酮类化合物。
