[猿猴病毒40感染及其在人脑肿瘤中的起源研究]
[A study of simian virus 40 infection and its origin in human brain tumors].
作者信息
Bu X, Zhang X, Zhang X, Yi S
机构信息
Department of Neurosurgery, Henan Provincial People's Hospital, Zhengzhou 450003, China.
出版信息
Zhonghua Liu Xing Bing Xue Za Zhi. 2000 Feb;21(1):19-21.
OBJECTIVE
To study the status of simian virus 40 (SV40) infection and its origin in human brain tumors.
METHODS
Polymerase chain reaction (PCR) and Dot blot hybridization were used to detect SV40 DNA sequences in 516 human brain tumor tissues, 80 peripheral blood cells and 50 sperm fluids from healthy individuals, 100 human embryo tissues from artificial abortion, 30 normal brain tissues and two human glioma cell lines: SHG44 and BT325.
RESULTS
SV40 DNA sequences were found in 36.4% of human brain tumors (188/516), 16.3% of healthy peripheral blood cells (13/80), 22.0% of healthy semen (11/50), 8.0% of human embryo tissues (8/100) and 6.7% of normal brain tissues (2/30). SV40 DNA sequences were also detected in SHG44 and BT325 cell lines. The positive rates of SV40 DNA in human brain tumors, peripheral blood cells and semen from healthy donors were significantly higher than those in human normal brain and embryo tissues (P < 0.05).
CONCLUSIONS
(1) SV40 has a higher infection rate in human brain tumors, (2) SV40 is closely related to the etiopathogenesis of human brain tumors, (3) the ways of SV40 spread may due to both horizontal and diaplacental infections in human population.
目的
研究猿猴病毒40（SV40）在人脑肿瘤中的感染状况及其来源。
方法
采用聚合酶链反应（PCR）和斑点杂交法检测516例人脑肿瘤组织、80例健康个体外周血细胞、50例健康个体精液、100例人工流产的人胚胎组织、30例正常脑组织以及两种人胶质瘤细胞系SHG44和BT325中的SV40 DNA序列。
结果
在36.4%的人脑肿瘤（188/516）、16.3%的健康外周血细胞（13/80）、22.0%的健康精液（11/50）、8.0%的人胚胎组织（8/100）和6.7%的正常脑组织（2/30）中发现了SV40 DNA序列。在SHG44和BT325细胞系中也检测到了SV40 DNA序列。人脑肿瘤、健康供体外周血细胞和精液中SV40 DNA的阳性率显著高于人正常脑和胚胎组织（P<0.05）。
结论
（1）SV40在人脑肿瘤中的感染率较高；（2）SV40与人脑肿瘤的发病机制密切相关；（3）SV40的传播途径可能是人群中的水平传播和经胎盘传播。
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