Wang L, Gu Y, Wu G, Wang W, Liu J, Liu J, Wu Z
Department of Atherosclerosis, Beijing Heart Lung and Blood Vessel Medical Institute, Beijing 100029, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2000 Feb;21(1):22-5.
To investigate the frequency of the restrict fragment length polimorphisms (RFLP) at 2 MspI sites in the 5' - end of apolipoprotein AI (ApoAI) gene and its potential interaction to atherosclerotic cerebral infarction (ACI) in Chinese Han population.
Polymerase chain reaction (PCR) technique was used in a sample of 199 cases with documented ACI and 204 healthy matched by age and sex individuals selected from Chinese Han nationality in Beijing. The studied loci include promoter region (-75 bp) and the intron - 1 (+83 bp).
(1) Both ACI and control groups M1 + + and M1 + - were major frequent genotypes in Beijng area, M2 + + were major frequent genotypes in intron - 1; (2) M1 + - genotype appeared more common among patients than in controls (0.487 vs 0.368, P < 0.05, OR = 1.64); (3) Individuals with M1 + -/M2 + + haplotype were significantly increased in patients compared with controls (0.437 vs 0.314, P < 0.05, OR = 1.70).
It is suggested that both M1 + - genotype and M1 + -/M2 + + haplotype of ApoAI gene might associate with ACI in our study.
研究载脂蛋白AI（ApoAI）基因5′端2个MspI位点的限制性片段长度多态性（RFLP）频率及其与中国汉族人群动脉粥样硬化性脑梗死（ACI）的潜在关系。
采用聚合酶链反应（PCR）技术，对199例确诊为ACI的患者及从北京汉族人群中选取的204例年龄和性别相匹配的健康个体进行研究。研究位点包括启动子区（-75bp）和内含子1（+83bp）。
（1）在北京地区，ACI组和对照组中M1++和M1+-均为主要常见基因型，内含子1中M2++为主要常见基因型；（2）M1+-基因型在患者中比在对照组中更常见（0.487对0.368，P<0.05，OR=1.64）；（3）与对照组相比，患者中M1+-/M2++单倍型个体显著增加（0.437对0.314，P<0.05，OR=1.70）。
在本研究中，提示ApoAI基因的M1+-基因型和M1+-/M2++单倍型可能与ACI相关。
