Harad Kumiko, Kuniyasu Akihiko, Nakayama Hitoshi, Nakayama Morio, Matsunaga Toshiro, Uji Yoshinori, Sugiuchi Hiroyuki, Okabe Hiroaki
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Feb 5;767(1):45-51. doi: 10.1016/s0378-4347(01)00529-1.
Four molecular forms of transferrins with different iron-binding states were separated by HPLC using a pyridinium polymer column. The elution order was monoferric transferrin bound to the C-site, holotransferrin, apotransferrin and monoferric transferrin bound to the N-site. Human sera were also analyzed with the column, and ICP-MS combined with HPLC was used to detect iron in each peak. Transferrin peaks separated by HPLC were also confirmed by an immunological method. The percentages of iron saturation in transferrins obtained by the HPLC method were compared with the values calculated from clinical data.
使用吡啶聚合物柱通过高效液相色谱法（HPLC）分离出具有不同铁结合状态的四种转铁蛋白分子形式。洗脱顺序为结合到C位点的单铁转铁蛋白、全转铁蛋白、脱铁转铁蛋白和结合到N位点的单铁转铁蛋白。还用该柱分析了人血清，并使用电感耦合等离子体质谱（ICP-MS）与HPLC联用检测每个峰中的铁。通过HPLC分离的转铁蛋白峰也通过免疫方法得到了证实。将通过HPLC方法获得的转铁蛋白中铁饱和度的百分比与根据临床数据计算的值进行了比较。
