Wang Y, Li Y, Zhu G, Wang X, Wu S, Zhang L, Gao X
Department of Pathology, Normen Bethune University of Medical Science, ChangChun 130021, China.
Zhonghua Bing Li Xue Za Zhi. 2000 Jun;29(3):176-9.
To study the morphology of pericyte, the relationship between pericytes and neovascularization of breast carcinoma.
Ultrastructural, immunohistochemical and quantitative morphology analysis techniques were used to study 89 cases of human breast carcinoma. 4 human granulation tissues cases were used as control.
The positive cells for FVIIIRAg were endothelial cells situated inside the vascular wall with abundant cytoplasm containing caveolae and Weibel-palade bodies. The positive cells for alpha-SMA were pericytes with cytoplasm containing abundant myofilament, and connections with endothelial cells. The pericyte-endothelial junctions can be found. Quantitative morphological analysis showed that the number of pericytes in the low-microvascular density areas are much higher than that in the high- microvascular density areas (P < 0.0005).
Ultrastructural observation and immunohistochemical staining of alpha-SMA are useful in identifying pericytes. The results of this study imply that pericytes may possibly inhibit angiogenesis.
研究周细胞的形态，以及周细胞与乳腺癌新生血管形成之间的关系。
采用超微结构、免疫组织化学及定量形态学分析技术对89例人乳腺癌进行研究。以4例人肉芽组织作为对照。
FVIIIRAg阳性细胞为位于血管壁内的内皮细胞，其胞质丰富，含有小凹和W-P小体。α-SMA阳性细胞为周细胞，其胞质含有丰富的肌丝，并与内皮细胞相连。可发现周细胞-内皮连接。定量形态学分析显示，低微血管密度区域的周细胞数量远高于高微血管密度区域（P<0.0005）。
超微结构观察及α-SMA免疫组织化学染色有助于识别周细胞。本研究结果提示周细胞可能抑制血管生成。
