Dan'ura Tomoyuki, Kawai Akira, Morimoto Yuki, Naito Noriko, Yoshida Aki, Inoue Hajime
Department of Orthopaedic Surgery, Faculty of Medicine, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
Cancer Lett. 2002 Apr 25;178(2):167-74. doi: 10.1016/s0304-3835(01)00836-9.
Information for the occurrence and extent of apoptosis in soft tissue sarcoma (STS) and their clinical implication are limited. In 102 cases of STSs, apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling method and expression of Bcl-2, Bax, MIB-1 and p53 protein was examined immunohistochemically. The apoptotic index of the STSs ranged from 0 to 15% with an average value of 1.9%. The mean values of positive cell staining for Bcl-2, Bax, MIB-1 and p53 protein were 32.1, 40.8, 17.0 and 20.3%, respectively. The extent of apoptosis and expression of Bcl-2 protein were correlated to the histologic types of the tumor. Synovial sarcoma had a significantly higher expression of Bcl-2 protein, and lower incidence of apoptosis. STS underwent apoptosis at a constitutional level. There were no significant relationships between extent of the apoptosis, expression of its regulatory proteins and prognosis of the patients.
关于软组织肉瘤（STS）中细胞凋亡的发生情况、程度及其临床意义的信息有限。在102例STS病例中，采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法检测细胞凋亡，并通过免疫组织化学法检测Bcl-2、Bax、MIB-1和p53蛋白的表达。STS的凋亡指数范围为0至15%，平均值为1.9%。Bcl-2、Bax、MIB-1和p53蛋白的阳性细胞染色平均值分别为32.1%、40.8%、17.0%和20.3%。细胞凋亡程度和Bcl-2蛋白表达与肿瘤的组织学类型相关。滑膜肉瘤中Bcl-2蛋白表达显著较高，细胞凋亡发生率较低。STS在组织水平上发生细胞凋亡。细胞凋亡程度、其调节蛋白表达与患者预后之间无显著关系。
