Chankvetadze Lali, Kartozia Irma, Yamamoto Chiyo, Chankvetadze Bezhan, Blaschke Gottfried, Okamoto Yoshio
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan.
Electrophoresis. 2002 Feb;23(3):486-93. doi: 10.1002/1522-2683(200202)23:3<486::AID-ELPS486>3.0.CO;2-L.
The potential of the widely used chiral stationary phase for high-performance liquid chromatography (HPLC) enantioseparations, cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC, sold under the trade name Chiralcel OD) was evaluated under the conditions of nonaqueous capillary electrochromatography (CEC). The effect of the particle size of the silica gel, the loading of CDMPC on the silica gel and nature of the organic solvent, as well as electrolyte salts on the separation characteristics were investigated. This study illustrates the applicability of CDMPC for obtaining highly efficient enantioseparations under the conditions of nonaqueous CEC. Comparative study of enantioseparations in capillary liquid chromatography (CLC) and CEC indicated the significant advantages of CEC such as higher plate number at the similar linear flow rates of the mobile phase as well as better tolerance of higher linear flow rates.
在非水毛细管电色谱（CEC）条件下，对广泛用于高效液相色谱（HPLC）对映体分离的手性固定相——三（3,5-二甲基苯基氨基甲酸酯）纤维素（CDMPC，商品名为Chiralcel OD）的潜力进行了评估。研究了硅胶粒径、CDMPC在硅胶上的负载量、有机溶剂的性质以及电解质盐对分离特性的影响。本研究表明了CDMPC在非水CEC条件下获得高效对映体分离的适用性。毛细管液相色谱（CLC）和CEC中对映体分离的对比研究表明了CEC的显著优势，如在相似的流动相线性流速下具有更高的塔板数，以及对更高线性流速具有更好的耐受性。
