Chankvetadze Bezhan, Yamamoto Chiyo, Tanaka Nobuo, Nakanishi Kazuki, Okamoto Yoshio
Molecular Recognition and Separation Science Laboratory, School of Chemistry, Tbilisi State University, Chavchavadze Ave 1, 380028 Tbilisi, Georgia.
J Sep Sci. 2004 Jul;27(10-11):905-11. doi: 10.1002/jssc.200401819.
Monolithic capillary columns containing native silica gel were modified with cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC) and used for enantioseparations in capillary liquid chromatography. The method adopted for in situ enantioselective modification of monolithic fused silica capillary columns by coating with CDPMC appears to be fairly simple and fast. High efficiency enantioseparations of test racemic compounds and s(everal chiral drugs were achieved in a short time. It was possible to increase the amount of chiral selector present by multiple coating of monoliths with CDMPC. The baseline enantioseparation of 2,2,2-trifluoro-1-(9-anthryl)ethanol was achieved in an analysis time less than 30 s with this capillary column. In addition, reproducible enantioseparations were obtained when the chiral selector was removed from the monolithic column by flushing it with appropriate solvent and the column recoated.
含有天然硅胶的整体式毛细管柱用三（3,5-二甲基苯基氨基甲酸酯）纤维素（CDMPC）进行修饰，并用于毛细管液相色谱中的对映体分离。通过用CDPMC涂覆对整体式熔融石英毛细管柱进行原位对映体选择性修饰所采用的方法似乎相当简单且快速。在短时间内实现了测试外消旋化合物和几种手性药物的高效对映体分离。通过用CDMPC对整体柱进行多次涂覆，可以增加手性选择剂的量。使用该毛细管柱在不到30秒的分析时间内实现了2,2,2-三氟-1-（9-蒽基）乙醇的基线对映体分离。此外，当用适当的溶剂冲洗整体柱并重新涂覆手性选择剂时，可获得可重现的对映体分离。
