通过菌落杂交检测青春双歧杆菌作为人类粪便污染的指标。
The detection of Bifidobacterium adolescentis by colony hybridization as an indicator of human faecal pollution.
作者信息
Lynch P A, Gilpin B J, Sinton L W, Savill M G
机构信息
Institute of Environmental Science and Research (ESR) Ltd., Christchurch Science Centre, PO Box 29-181, Christchurch, New Zealand.
出版信息
J Appl Microbiol. 2002;92(3):526-33. doi: 10.1046/j.1365-2672.2002.01557.x.
AIMS
To develop an improved method for the detection of Bifidobacterium adolescentis as an indicator of human faecal pollution.
METHODS AND RESULTS
Bifidobacterium medium (BFM) was identified as the optimal medium for the recovery of bifidobacteria from human effluent. Dilutions of faeces and effluent from both humans and animals were filtered, grown on BFM and human specific B. adolescentis identified via colony hybridization with a digoxigenin (DIG)-labelled oligonucleotide probe.
CONCLUSIONS
The combination of BFM with colony probing allows the detection of B. adolescentis, a specific indicator of human faecal pollution.
SIGNIFICANCE AND IMPACT OF THE STUDY
It is now technically feasible to use B. adolescentis as indicators of human faecal pollution, and studies to examine the survival and appropriateness of bifidobacteria in this role can be initiated.
目的
开发一种改进的方法来检测青春双歧杆菌，以此作为人类粪便污染的指标。
方法与结果
双歧杆菌培养基（BFM）被确定为从人类污水中回收双歧杆菌的最佳培养基。对人类和动物的粪便及污水稀释液进行过滤，在BFM上培养，并通过与地高辛（DIG）标记的寡核苷酸探针进行菌落杂交来鉴定人类特有的青春双歧杆菌。
结论
BFM与菌落探针相结合能够检测出青春双歧杆菌，它是人类粪便污染的一种特异性指标。
研究的意义与影响
现在将青春双歧杆菌用作人类粪便污染指标在技术上是可行的，并且可以启动相关研究来检验双歧杆菌在这一角色中的存活情况及适用性。
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