选择性培养法会低估双歧杆菌的数量，且显示出从人类粪便中回收双歧杆菌物种的差异。
Selective plating underestimates abundance and shows differential recovery of bifidobacterial species from human feces.
作者信息
Apajalahti Juha H A, Kettunen Anu, Nurminen Päivi H, Jatila Hanna, Holben William E
机构信息
Enteromix Research, Danisco Innovation, FIN-02460 Kantvik, Finland.
出版信息
Appl Environ Microbiol. 2003 Sep;69(9):5731-5. doi: 10.1128/AEM.69.9.5731-5735.2003.
Abstract
The aim of the present work was to compare the efficacies and levels of selectivity of different culture-dependent and -independent methods for analyzing bifidobacteria in human stool samples. The three different culture media used here significantly differed from each other, particularly with regard to the recovery of Bifidobacterium adolescentis. Bifidobacterium medium failed to recover B. adolescentis; Beerens medium recovered some B. adolescentis organisms (17% of total bifidobacteria), whereas tomato-Eugon medium recovered mainly B. adolescentis organisms (58% of total bifidobacteria). A culture-independent method that combines GC fractionation of bacterial community DNA and 16S rRNA sequencing indicated that B. adolescentis organisms accounted for 85% of all bifidobacteria. Methodological biases, such as those described in this paper, should be taken into account in interpreting earlier studies and designing future experiments.
摘要
本研究的目的是比较不同的依赖培养法和非依赖培养法在分析人类粪便样本中双歧杆菌时的效能和选择性水平。这里使用的三种不同培养基彼此间存在显著差异，尤其是在青春双歧杆菌的回收率方面。双歧杆菌培养基未能回收青春双歧杆菌；贝伦斯培养基回收了一些青春双歧杆菌菌株（占双歧杆菌总数的17%），而番茄-尤贡培养基主要回收了青春双歧杆菌菌株（占双歧杆菌总数的58%）。一种将细菌群落DNA的气相色谱分离与16S rRNA测序相结合的非依赖培养法表明，青春双歧杆菌菌株占所有双歧杆菌的85%。在解释早期研究和设计未来实验时，应考虑到本文所述的方法偏差。
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2
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