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1
Isolation and in vitro translation of human placental lactogen messenger RNA from human term placenta.从足月人胎盘中分离人胎盘催乳素信使核糖核酸并进行体外翻译
Nucleic Acids Res. 1975 Oct;2(10):1903-10. doi: 10.1093/nar/2.10.1903.
2
Synthesis of human placental lactogen and human chorionic gonadotropin by polyribosomes and messenger RNA's from early and full term placentas.利用来自早期和足月胎盘的多核糖体及信使核糖核酸合成人胎盘催乳素和人绒毛膜促性腺激素。
J Biol Chem. 1976 May 25;251(10):2945-51.
3
Properties of biologically active messenger RNA from human placenta. Cell-free synthesis of two immunoreactive forms of placental lactogen.人胎盘生物活性信使核糖核酸的特性。胎盘催乳素两种免疫反应形式的无细胞合成。
J Biol Chem. 1976 Mar 25;251(6):1723-30.
4
The synthesis of human placental lactogen hormone (hPL) in a cell-free wheat germ system.人胎盘催乳素（hPL）在无细胞小麦胚芽系统中的合成。
Mol Biol Rep. 1976 Nov;3(2):189-93. doi: 10.1007/BF00423234.
5
Synthesis of human placental lactogen messenger RNA as a function of gestation.人胎盘催乳素信使核糖核酸的合成与妊娠的关系。
J Biol Chem. 1976 Feb 10;251(3):820-5.
6
Comparisons of human placental lactogen mRNA levels from placentas of diabetics and normal term.糖尿病患者与足月正常孕妇胎盘的人胎盘催乳素mRNA水平比较。
Mol Cell Endocrinol. 1985 Jan;39(1):61-9. doi: 10.1016/0303-7207(85)90092-9.
7
The synthesis of human placental lactogen by ribosomes derived from human placenta.源自人胎盘的核糖体合成人胎盘催乳素。
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1322-5. doi: 10.1073/pnas.71.4.1322.
8
Distribution, quantification and biological activity of messenger RNA coding for human chorionic somatomammotropin during normal pregnancy.正常妊娠期间编码人绒毛膜生长催乳素的信使核糖核酸的分布、定量及生物活性
Mol Cell Endocrinol. 1981 Dec;24(3):339-55. doi: 10.1016/0303-7207(81)90008-3.
9
Synthesis of human placental lactogen in Xenopus oocytes.非洲爪蟾卵母细胞中人胎盘催乳素的合成。
Mol Biol Rep. 1976 Apr;2(5):407-12. doi: 10.1007/BF00366263.
10
Membrane-dependent cleavage of the human placental lactogen precursor to its native form in ascites cell-free extracts.在腹水无细胞提取物中，人胎盘催乳素前体向其天然形式的膜依赖性切割。
Eur J Biochem. 1977 Mar 1;73(2):515-20. doi: 10.1111/j.1432-1033.1977.tb11345.x.

引用本文的文献

1
Translation in a cell-free system of mRNA from term placenta extracted by guanidine hydrochloride.在无细胞体系中对用盐酸胍提取的足月胎盘mRNA进行翻译。
Mol Biol Rep. 1983 Aug;9(3):203-8. doi: 10.1007/BF00775369.
2
Synthesis of human placental lactogen in Xenopus oocytes.非洲爪蟾卵母细胞中人胎盘催乳素的合成。
Mol Biol Rep. 1976 Apr;2(5):407-12. doi: 10.1007/BF00366263.
3
The synthesis of human placental lactogen hormone (hPL) in a cell-free wheat germ system.人胎盘催乳素（hPL）在无细胞小麦胚芽系统中的合成。
Mol Biol Rep. 1976 Nov;3(2):189-93. doi: 10.1007/BF00423234.

本文引用的文献

1
Ribosomes in rat liver: an estimate of the percentage of free and membrane-bound ribosomes interacting with messenger RNA in vivo.大鼠肝脏中的核糖体：体内游离核糖体和膜结合核糖体与信使核糖核酸相互作用的百分比估算
J Mol Biol. 1967 Sep 28;28(3):539-42. doi: 10.1016/s0022-2836(67)80103-7.
2
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
3
Characteristics of RNA degradation in the erythroid cell.红细胞中RNA降解的特征。
J Clin Invest. 1969 Jul;48(7):1266-72. doi: 10.1172/JCI106092.
4
Serum placental lactogen (HPL) levels as an index of placental function.血清胎盘催乳素（HPL）水平作为胎盘功能的一项指标。
N Engl J Med. 1969 Jul 31;281(5):225-31. doi: 10.1056/NEJM196907312810501.
5
Molecular weight of human placental lactogen investigated by gel filtration.通过凝胶过滤法研究人胎盘催乳素的分子量。
Biochem J. 1969 Mar;111(5):799-800. doi: 10.1042/bj1110799.
6
Prolonged nutritional deprivation in pregnancy: changes in human chorionic somatomammotropin and growth hormone secretion.孕期长期营养缺乏：人绒毛膜生长催乳素与生长激素分泌的变化
Am J Obstet Gynecol. 1971 Apr 1;109(7):1080-2. doi: 10.1016/0002-9378(71)90293-6.
7
Biosynthesis of human placental proteins and human placental lactogen (HPL) in vitro. II. Dynamic studies of normal term placentas.人胎盘蛋白和人胎盘催乳素（HPL）的体外生物合成。II. 足月正常胎盘的动态研究。
Endocrinology. 1969 Dec;85(6):1037-45. doi: 10.1210/endo-85-6-1037.
8
A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.在小鼠肉瘤180腹水细胞快速标记的多核糖体RNA组分中富含腺苷酸的多核苷酸片段。
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331-5. doi: 10.1073/pnas.68.6.1331.
9
The molecular properties of human chorionic somatomammotropin.人绒毛膜生长催乳素的分子特性
J Biol Chem. 1971 Aug 25;246(16):5047-52.
10
Isolation and properties of polysomes from human placenta.人胎盘多核糖体的分离及特性
Biochim Biophys Acta. 1970 Aug 8;213(2):391-400. doi: 10.1016/0005-2787(70)90047-x.

从足月人胎盘中分离人胎盘催乳素信使核糖核酸并进行体外翻译

Isolation and in vitro translation of human placental lactogen messenger RNA from human term placenta.

作者信息

Hubert C, Cedard L

出版信息

Nucleic Acids Res. 1975 Oct;2(10):1903-10. doi: 10.1093/nar/2.10.1903.

DOI:10.1093/nar/2.10.1903
PMID:1187349
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343555/
Abstract

A messenger activity for HPL was identified in normal human term placentas. The mRNA was translated in rabbit reticulocyte cell-free system. The HPL synthesized was quantified by a specific immunoprecipitation and further identified by electrophoresis on sodium dodecyl sulfate polyacrylamide gel. The HPL synthesized in the reticulocyte lysate exhibited a molecular weight between 20,000 and 22,000 daltons similar to the active hormone. The messenger RNA activity for HPL corresponded to a sedimentation coefficient of 11-12 S. Furthermore the messenger activity for HPL was preferentially associated with membrane bound polyribosomes than with free polyribosomes.

摘要

在正常足月人胎盘中鉴定出了人胎盘催乳素（HPL）的信使活性。该信使核糖核酸（mRNA）在兔网织红细胞无细胞系统中进行翻译。通过特异性免疫沉淀对合成的HPL进行定量，并通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳进一步鉴定。在网织红细胞裂解物中合成的HPL表现出与活性激素相似的20,000至22,000道尔顿的分子量。HPL的信使核糖核酸活性对应于11 - 12 S的沉降系数。此外，HPL的信使活性优先与膜结合多核糖体相关，而非游离多核糖体。