使用聚丙烯酰胺凝胶对火鸡血清碱性磷酸酶同工酶进行圆盘电泳。2. 火鸡血清碱性磷酸酶酶谱
Disc electrophoresis of turkey serum alkaline phosphatase isoenzymes using polyacrylamide gel. 2. Turkey serum alkaline phosphatase zymograms.
作者信息
Beck J R, Coleman T H, Wolford J H
出版信息
Poult Sci. 1975 Sep;54(5):1366-71. doi: 10.3382/ps.0541366.
The isoenzymes of alkaline phosphatase (akp) in turkey serum were separated by disc electrophoresis using polyacrylamide gel as the supporting medium Test subjects consisted of 103 turkeys maintained for the "Vibrator" condition at Michigan State University; and 46 female turkey of the Nicholas Broad Breasted White egg laying strain. Thirteen distinct akp bands were isolated. The 13 bands were observed to occur in 13 different patterns, each pattern being classified as a separate zymogram. Specific types of zymograms were detected for "Vibrator" birds vs. Nicholas strain; females vs, males; high intensity egg production vs. low intensity egg production and molting vs. laying.
以聚丙烯酰胺凝胶作为支持介质，通过圆盘电泳对火鸡血清中的碱性磷酸酶（akp）同工酶进行分离。实验对象包括在密歇根州立大学维持“振动器”条件下饲养的103只火鸡，以及46只尼古拉斯宽胸白羽产蛋品系的雌性火鸡。分离出了13条不同的akp条带。观察到这13条带以13种不同模式出现，每种模式被归类为一个单独的酶谱。检测到了特定类型的酶谱，分别用于比较“振动器”饲养的火鸡与尼古拉斯品系；雌性与雄性；高强度产蛋与低强度产蛋以及换羽期与产蛋期。
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