May Sean T, Clements Deborah, Bennett Malcolm J
Nottingham Arabidopsis Stock Centre, School of Biosciences, Plant Science Division, University of Nottingham, UK.
Mol Biotechnol. 2002 Feb;20(2):209-21. doi: 10.1385/MB:20:2:209.
The process of finding a mutant plant for your gene of interest has recently become far more straightforward and painless than has ever been possible before. This has come about through the production of large-scale insertional mutagenesis populations. These can now be readily screened in bulk for insertional mutants through a mixture of molecular (PCR/hybridization) and bioinformation techniques. Here we describe a step-by-step guide to the molecular protocols and a description of the bioinformatics approach.
寻找感兴趣基因的突变植物的过程，近来已变得比以往任何时候都更加直接和轻松。这是通过大规模插入诱变群体的产生得以实现的。现在，可以通过分子（PCR/杂交）和生物信息学技术的组合，对这些群体进行批量筛选以找出插入突变体。在此，我们描述分子实验方案的分步指南以及生物信息学方法的介绍。
