Ko C H, Brendel V, Taylor R D, Walbot V
Department of Biologìcal Sciences, Stanford University, CA 94305-5020, USA.
Plant Mol Biol. 1998 Mar;36(4):573-83. doi: 10.1023/a:1005932620374.
Using a large set of plant gene sequences we compared individual introns to their flanking exons. Both Zea mays and Arabidopsis thaliana introns are U-rich but display no apparent bias for A. We identified fifteen 11-mer U-rich motifs as frequent elements of maize introns, and these are virtually absent from exons. By mutagenesis, we show that the single U-rich motif in the Bronze2 intron of maize plays a key role in intron processing in vivo.
我们使用大量植物基因序列，将单个内含子与其侧翼外显子进行了比较。玉米和拟南芥的内含子都富含尿嘧啶（U），但对腺嘌呤（A）没有明显偏好。我们鉴定出15个富含U的11聚体基序，它们是玉米内含子的常见元件，而在外显子中几乎不存在。通过诱变，我们表明玉米Bronze2内含子中的单个富含U的基序在体内内含子加工中起关键作用。
