Yamada T, Onda M, Tanaka N
First Dept. of Surgery, Nippon Medical School, Sendagi, Tokyo, Japan.
J Exp Clin Cancer Res. 2001 Dec;20(4):561-7.
Human colorectal neuroendocrine cell carcinoma ( NECC ) is uncommon. Treatment of the disease has not yet been established, and NECC of the colon and rectum behave clinically more aggressively than their exocrine counterparts, so the prognosis is generally worse. One reason for the lack of established treatment is that there are no model systems of this disease. There have been a few reports on cell lines from neuroendocrine tumors, because these tumors are difficult to culture, and there are even fewer reports on colorectal carcinoma cell lines with neuroendocrine features. We therefore attempted to establish a permanent cell line in order to investigate the biological behavior and treatment of NECC. The cell line we succeeded in culturing is called N-TAK1. Gastrin promotes the growth of gastrointestinal epithelial cells and also stimulates the growth of gastrointestinal cancers. Hormone-receptor antagonists restrict the growth of hormone-dependent tumors. The growth of colon cancer was promoted by the application of gastrin, whereas it was restricted by proglumide, which is known to be a gastrin receptor antagonist. We demonstrated that gastrin has a stimulatory effect on the growth of N-TAK1 cells and that it could be detected by immunohistochemistry in the cells. We also showed that proglumide inhibited the growth effect of gastrin.
人类结直肠神经内分泌细胞癌（NECC）并不常见。该疾病的治疗方法尚未确立，而且结肠和直肠的NECC在临床上比其外分泌对应物表现得更具侵袭性，因此总体预后更差。缺乏既定治疗方法的一个原因是没有这种疾病的模型系统。关于神经内分泌肿瘤细胞系已有一些报道，因为这些肿瘤难以培养，而具有神经内分泌特征的结直肠癌细胞系的报道则更少。因此，我们试图建立一个永久细胞系，以研究NECC的生物学行为和治疗方法。我们成功培养的细胞系称为N-TAK1。胃泌素可促进胃肠道上皮细胞的生长，也能刺激胃肠道癌症的生长。激素受体拮抗剂可限制激素依赖性肿瘤的生长。应用胃泌素可促进结肠癌的生长，而已知作为胃泌素受体拮抗剂的丙谷胺则可限制其生长。我们证明胃泌素对N-TAK1细胞的生长有刺激作用，并且可以通过免疫组织化学在细胞中检测到。我们还表明丙谷胺可抑制胃泌素的生长作用。
