粉防己碱对胶原合成及瘢痕成纤维细胞DNA的影响

[Effects of tetrandrine on the synthesis of collagen and scar-derived fibroblast DNA].

作者信息

Liu D, Li G, Liu D, Cao Y

机构信息

Department of Burns, The First Affiliated Hospital, Jiang Xi Medical College, Nanchang 330006, Jiang Xi Province, P. R. China.

出版信息

Zhonghua Shao Shang Za Zhi. 2001 Aug;17(4):222-4.

PMID:11876945

Abstract

OBJECTIVE

To investigate the effects of tetrandrine on the synthesis of collagen and scar derived fibroblast DNA.

METHODS

Scar-derived human fibroblasts were cultured in vitro. The changes in the levels of the synthesis of collagen and DNA of the fibroblasts were represented by the incorporation values of (3)H-TdR and (3)H-proline into the cells. Tetrandrine was added to the culture media of the cells, and its effects were studied.

RESULTS

When the concentration of added tetrandrine increased from 5 mg/L to 80 mg/L, The (3)H-TdR values in scar-derived fibroblasts were 1162 plus minus 226 and 412 plus minus 82, respectively while that in control group was 1740 plus minus 165, showing an inhibition rate of 76.32%, and the difference (P < 0.01). In addition, the (3)H-proline incorporation values in the cells were 535 plus minus 141 and 341 plus minus 89, respectively, while that in control group was 1126 plus minus 193, with the inhibition rate of 69.71%, showing significant difference (P < 0.01).

CONCLUSION

The synthesis of DNA and collagen in cultured scar-derived fibroblasts could be inhibited by tetrandrine in dose-dependent pattern. Tetrandrine might be a potential agent for the prevention and treatment of proliferative scars.

摘要

目的

研究粉防己碱对瘢痕成纤维细胞胶原蛋白合成及DNA的影响。

方法

体外培养瘢痕来源的人成纤维细胞。以（3）H-TdR和（3）H-脯氨酸掺入细胞的量来表示成纤维细胞胶原蛋白及DNA合成水平的变化。向细胞培养基中加入粉防己碱并研究其作用。

结果

当粉防己碱添加浓度从5mg/L增至80mg/L时，瘢痕来源成纤维细胞的（3）H-TdR值分别为1162±226和412±82，而对照组为1740±165，抑制率为76.32%，差异有统计学意义（P<0.01）。此外，细胞中（3）H-脯氨酸掺入值分别为535±141和341±89，而对照组为1126±193，抑制率为69.71%，差异有统计学意义（P<0.01）。