[Blocking action of tetrandrine on the cell proliferation induced by PDGF in human lung fibroblasts and liver Ito cell of rats].

OBJECTIVE

To investigate the mechanism of hepatic fiberosis, the effects of platelet-derived growth factor (PDGF) on cell proliferation activity and collagen synthesis in human lung fibroblasts (HLF) and Ito cells of rat liver as well as the blocking action of tetrandrine on the cell proliferation induced by PDGF.

METHODS

The Ito cells were separated from liver of rats by using the perfusion of collagenase and density pradient centrifugation. The cell proliferation was observed with the incorporation method of H-TdR and 3H-L-proline.

RESULTS

Under condition of cell culture with 1% serum, PDGF could significantly enhance DNA synthesis, cell proliferation and collagen synthesis, which increased in degree with a rising of concentration of PDGF. This occurred in culture without serum, but under the same condition, if PDGF was applied together with insulin-like growth factor (IGF), it enhanced cell proliferation. The result also showed that the dose of tetrandrine below the level of inhibiting cell DNA synthesis could antagonize the stimulation action of PDGF on cell proliferation and collagen synthesis.

CONCLUSION

Tetrandrine plays the blocking action in the promoting cell proliferation induced by PDGF.