Suzuki Genki, Yanagawa Yuki, Kwok Shing F, Matsui Minami, Deng Xing-Wang
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
Genes Dev. 2002 Mar 1;16(5):554-9. doi: 10.1101/gad.964602.
A group of evolutionarily conserved pleiotropic COP/DET/FUS proteins was initially defined by their ability to repress photomorphogenesis in Arabidopsis. It was proposed that this regulation be mediated by targeting degradation of key cellular regulators that promote photomorphogenesis. Among them, COP1 and the COP9 signalosome have been hypothesized to fulfill the roles as an ubiquitin ligase (E3) and an essential E3 modulator. Here we report that COP10 encodes a protein similar to ubiquitin-conjugating enzyme (E2) variant proteins (UEV). COP10 is part of a nuclear protein complex and capable of directly interacting with both COP1 and the COP9 signalosome. Our data indicates that COP10 defines a possible E2 activity, thus validating the working hypothesis that the pleiotropic COP/DET/FUS group of proteins defined a protein ubiquitination pathway.
一组进化上保守的多效性COP/DET/FUS蛋白最初是根据它们在拟南芥中抑制光形态建成的能力来定义的。有人提出，这种调节是通过靶向降解促进光形态建成的关键细胞调节因子来介导的。其中，COP1和COP9信号体被假设分别发挥泛素连接酶（E3）和必需的E3调节剂的作用。在此，我们报道COP10编码一种类似于泛素结合酶（E2）变体蛋白（UEV）的蛋白质。COP10是一种核蛋白复合物的组成部分，能够直接与COP1和COP9信号体相互作用。我们的数据表明，COP10定义了一种可能的E2活性，从而验证了多效性COP/DET/FUS蛋白组定义了一条蛋白质泛素化途径的工作假设。
