Sahu Gautam Kumar, Tai Pei-Ching, Chatterjee Soma Banerjee, Lin Min-Hui, Tennant Bud, Gerin John, Shih Chiaho
Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0609, USA.
Virology. 2002 Jan 5;292(1):35-43. doi: 10.1006/viro.2001.1228.
Human hepatitis B virus (HBV) variants containing in-frame core internal deletion (CID) have been demonstrated to contain all the functional features of defective interfering (DI) particles (Yuan, T. T.-T., M.-H. Lin, D. S. Chen, and C. Shih, 1998, J. Virol. 72, 578-584). Here, we report that out-of-frame HBV CID variants exhibit defective interfering property similar to in-frame CID variants characterized previously. This result raises the possibility that it may be the deleted pregenomic RNA product, rather than the deleted core protein product, that is responsible for interference. Furthermore, a genomic deletion elsewhere does not cause interference since preS2 deletion variants exhibit no influence on wild-type HBV replication. Consistent with the natural occurrence of HBV CID variants, we recently identified CID variants of woodchuck hepatitis virus (WHV) in natural infection. However, unlike HBV CID variants, functional characterization of WHV CID variants using a human hepatoma cell line has not revealed any interference in tissue culture. In summary, defective interference is a general phenomenon for both in-frame and out-of-frame HBV CID variants.
已证实含有框内核心内部缺失（CID）的人乙型肝炎病毒（HBV）变体具有缺陷干扰（DI）颗粒的所有功能特征（Yuan，T.T.-T.，M.-H.Lin，D.S.Chen和C.Shih，1998年，《病毒学杂志》72卷，578 - 584页）。在此，我们报告框外HBV CID变体表现出与先前表征的框内CID变体相似的缺陷干扰特性。这一结果增加了一种可能性，即可能是缺失的前基因组RNA产物而非缺失的核心蛋白产物导致了干扰。此外，由于前S2缺失变体对野生型HBV复制没有影响，其他位置的基因组缺失不会引起干扰。与HBV CID变体的自然发生情况一致，我们最近在自然感染中鉴定出土拨鼠肝炎病毒（WHV）的CID变体。然而，与HBV CID变体不同，使用人肝癌细胞系对WHV CID变体进行功能表征并未在组织培养中发现任何干扰。总之，缺陷干扰是框内和框外HBV CID变体的普遍现象。
