The aim of this study was to explore individual and occupational factors possibly related to sensory thresholds in 484 middle-aged men and women from the general population. Multivariate analyses were performed using a linear model including eight covariates (age, body height, skin temperature, smoking habits, musculoskeletal symptoms during the last week before examination, isometric muscle strength, aerobic capacity, and average physical work load during the last 15 years). Men and women were considered separately in all analyses. Major findings were as follows: pressure pain thresholds increased with muscle strength; cold perception thresholds on the foot improved with increasing skin temperature; vibration and warm perception thresholds on the foot increased with age and body height; pressure pain threshold on the leg was decreased and vibration threshold on the hand was increased in women with musculoskeletal symptoms; pressure pain thresholds were slightly increased in men reporting long-lasting high physical work load. Significant sex differences were found for the majority of sensory thresholds, that is higher thresholds in men. However, body height was found to be more important than gender for differences in vibration and warm perception thresholds on the foot.