Henry Alyssa C, Waddell Emanuel A, Shreiner Rubina, Locascio Laurie E
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8394, USA.
Electrophoresis. 2002 Mar;23(5):791-8. doi: 10.1002/1522-2683(200203)23:5<791::AID-ELPS791>3.0.CO;2-K.
The fabrication of microchannels in poly(ethylene terephthalate glycol) (PETG) by laser ablation and the hot imprinting method is described. In addition, hot imprinted microchannels were hydrolyzed to yield additional charged organic functional groups on the imprinted surface. The charged groups are carboxylate moieties that were also used as a means for the further reaction of different chemical species on the surface of the PETG microchannels. The microchannels were characterized by fluorescence mapping and electroosmotic flow (EOF) measurements. Experimental results demonstrated that different fabrication and channel treatment protocols resulted in different EOF rates. Laser-ablated channels had similar EOF rates (5.3+/-0.3 x 10(-4) cm(2)/Vs and 5.6+/-0.4 x 10(-4) cm(2)/Vs) to hydrolyzed imprinted channels (5.1+/-0.4 x 10(-4) cm(2)/Vs), which in turn demonstrated a somewhat higher flow rate than imprinted PETG channels that were not hydrolyzed (3.5+/-0.3 x 10(-4) cm(2)/Vs). Laser-ablated channels that had been chemically modified to yield amines displayed an EOF rate of 3.38+/- 0.1 x 10(-4) cm(2)/Vs and hydrolyzed imprinted channels that had been chemically derivatized to yield amines showed an EOF rate of 2.67+/-0.6 cm(2)/Vs. These data demonstrate that surface-bound carboxylate species can be used as a template for further chemical reactions in addition to changing the EOF mobility within microchannels.
描述了通过激光烧蚀和热压印法在聚对苯二甲酸乙二酯二醇（PETG）中制备微通道的过程。此外，对热压印的微通道进行水解，以在压印表面产生额外的带电有机官能团。这些带电基团是羧酸盐部分，也用作PETG微通道表面上不同化学物质进一步反应的一种手段。通过荧光映射和电渗流（EOF）测量对微通道进行了表征。实验结果表明，不同的制备和通道处理方案导致不同的EOF速率。激光烧蚀通道的EOF速率（5.3±0.3×10⁻⁴ cm²/Vs和5.6±0.4×10⁻⁴ cm²/Vs）与水解压印通道（5.1±0.4×10⁻⁴ cm²/Vs）相似，而水解压印通道的流速又比未水解的压印PETG通道（3.5±0.3×10⁻⁴ cm²/Vs）略高。经过化学修饰以产生胺的激光烧蚀通道的EOF速率为3.38±0.1×10⁻⁴ cm²/Vs，经过化学衍生以产生胺的水解压印通道的EOF速率为2.67±0.6 cm²/Vs。这些数据表明，表面结合的羧酸盐物种除了可以改变微通道内的EOF迁移率外，还可以用作进一步化学反应的模板。
