Hoerauf Hans, Winkler Jörg, Scholz Christian, Wirbelauer Christopher, Gordes Roswitha S, Koch Peter, Engelhardt Ralf, Laqua Horst, Birngruber Reginald
Department of Ophthalmology, Medical University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany.
Lasers Surg Med. 2002;30(3):209-15. doi: 10.1002/lsm.10031.
To evaluate the potentials of a 1310-nm optical coherence tomography (OCT) system to penetrate the highly backscattering sclera in enucleated human eyes and provide visualization of intraocular structures by transscleral imaging.
STUDY DESIGN/MATERIALS AND METHODS: OCT-images were generated by an experimental prototype (Medical Laser Center, Lübeck, Germany) using a superluminescence diode with a wavelength of 1310 nm. OCT-images were taken from two enucleated human eyes using 100-200 axial scans with 60 Hz line scan frequency and compared to subsequent histologic sections.
Transscleral OCT allowed penetration of the sclera and the anterior chamber angle could be completely identified. Some change within the anterior eye segment could be demonstrated with high accuracy. Additionally, limited demonstration of the ciliary body region was achieved. Due to limited signal intensity no detailed imaging of the pars plana and pars plicata region was possible. However, more posterior measurements allowed transscleral visualization of a retinal detachment.
OCT using lightsources with a wavelength longer than that used in conventional OCT provides a promising imaging technique at high resolution allowing transscleral imaging of the anterior eye segment.
评估1310纳米光学相干断层扫描（OCT）系统穿透摘除的人眼高散射巩膜并通过经巩膜成像提供眼内结构可视化的潜力。
研究设计/材料与方法：使用波长为1310纳米的超发光二极管，由一个实验原型（德国吕贝克医学激光中心）生成OCT图像。对两只摘除的人眼进行OCT成像，采用60赫兹线扫描频率进行100 - 200次轴向扫描，并与后续的组织学切片进行比较。
经巩膜OCT能够穿透巩膜，前房角可被完全识别。眼前节内的一些变化能够高精度地显示出来。此外，睫状体区域的显示有限。由于信号强度有限，无法对扁平部和皱襞部区域进行详细成像。然而，更靠后的测量能够实现经巩膜对视网膜脱离的可视化。
使用波长比传统OCT更长的光源的OCT提供了一种有前景的高分辨率成像技术，允许对眼前节进行经巩膜成像。
