González-Billault C, Engelke M, Jiménez-Mateos E M, Wandosell F, Cáceres A, Avila J
Centro de Biología Molecular Severo Ochoa, CSIC, Universidad Autónoma de Madrid, Campus Cantoblanco, Madrid, Spain.
J Neurosci Res. 2002 Mar 15;67(6):713-9. doi: 10.1002/jnr.10161.
Several lines of evidence have indicated that changes in the structure of neuronal cytoskeleton provide the support for the dramatic morphological changes that occur during neuronal differentiation. It has been proposed that microtubule-associated proteins can contribute to the development of this phenomenon by controlling the dynamic properties of microtubules. In this report we have characterized the effect of the combined suppression of MAP1B and tau, and MAP1B and MAP2 on neuronal polarization in cultured hippocampal cells grown on a laminin-containing substrate. We have taken advantage of the use of a mouse line deficient in MAP1B expression obtained by the gene trapping approach. In addition to this engineered mice line we used the antisense oligonucleotide approach to induce the suppression of tau or MAP2, in wild type and MAP1B-deficient neurons. Together these results show a synergistic role for MAP1B/MAP2 and MAP1B/TAU.
多条证据表明，神经元细胞骨架结构的变化为神经元分化过程中发生的显著形态变化提供了支持。有人提出，微管相关蛋白可通过控制微管的动态特性促进这一现象的发展。在本报告中，我们描述了在含层粘连蛋白的底物上培养的海马细胞中，联合抑制MAP1B和tau以及MAP1B和MAP2对神经元极化的影响。我们利用了通过基因捕获方法获得的MAP1B表达缺陷小鼠品系。除了这种基因工程小鼠品系外，我们还使用反义寡核苷酸方法在野生型和MAP1B缺陷型神经元中诱导tau或MAP2的抑制。这些结果共同表明了MAP1B/MAP2和MAP1B/TAU的协同作用。
