Morakabati Payam, Assarehzadegan Mohammad-Ali, Khosravi Gholam Reza, Akbari Bahareh, Dousti Fatemeh
Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 1478736714, Iran.
Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 1478736714, Iran; Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
J Allergy (Cairo). 2016;2016:4092817. doi: 10.1155/2016/4092817. Epub 2016 Jan 26.
Sensitisation to Amaranthus retroflexus pollen is very common in tropical and subtropical countries. In this study we aimed to produce a recombinant allergenic Ole e 1-like protein from the pollen of this weed. To predict cross-reactivity of this allergen (Ama r 1) with other members of the Ole e 1-like protein family, the nucleotide sequence homology of the Ama r 1 was investigated. The expression of Ama r 1 in Escherichia coli was performed by using a pET-21b(+) vector. The IgE-binding potential of recombinant Ama r 1 (rAma r 1) was evaluated by immunodetection and inhibition assays using 26 patients' sera sensitised to A. retroflexus pollen. The coding sequence of the Ama r 1 cDNA indicated an open reading frame of 507 bp encoding for 168 amino acid residues which belonged to the Ole e 1-like protein family. Of the 26 serum samples, 10 (38.46%) had significant specific IgE levels for rAma r 1. Immunodetection and inhibition assays revealed that the purified rAma r 1 might be the same as that in the crude extract. Ama r 1, the second allergen from the A. retroflexus pollen, was identified as a member of the family of Ole e 1-like protein.
对反枝苋花粉过敏在热带和亚热带国家非常普遍。在本研究中，我们旨在从这种杂草的花粉中制备一种重组变应原Ole e 1样蛋白。为了预测这种变应原（Ama r 1）与Ole e 1样蛋白家族其他成员的交叉反应性，我们研究了Ama r 1的核苷酸序列同源性。利用pET-21b(+)载体在大肠杆菌中表达Ama r 1。使用26例对反枝苋花粉致敏患者的血清，通过免疫检测和抑制试验评估重组Ama r 1（rAma r 1）的IgE结合潜力。Ama r 1 cDNA的编码序列显示一个507 bp的开放阅读框，编码168个氨基酸残基，属于Ole e 1样蛋白家族。在26份血清样本中，10份（38.46%）对rAma r 1有显著的特异性IgE水平。免疫检测和抑制试验表明，纯化的rAma r 1可能与粗提物中的相同。Ama r 1是反枝苋花粉中的第二种变应原，被鉴定为Ole e 1样蛋白家族的成员。
