Nakashima T, Yano G, Masuda A, Uemura T, Morita M
Department of Otolaryngology, National Kyushu Cancer Center, Notame 3-1-1, Minami-ku, Fukuoka 815, Japan.
Eur Arch Otorhinolaryngol. 1994;251 Suppl 1:S76-9. doi: 10.1007/BF02565226.
Nucleolar organizer regions (NORs) were studied in surgically removed specimens from 76 patients with squamous cell carcinomas of the larynx using an argyrophilic (Ag) staining technique. The mean number of AgNORs per nucleus was 4.3 +/- 1.38 (SD). The mean AgNOR number for T1 or T2 disease was statistically lower than that for T3 or T4 lesions (P < 0.05). The mean AgNOR number was lower in patients with N0 disease than in patients with N1 or N2 or N3 tumors (P < 0.05). There was also a statistically significant difference between the mean AgNOR number for stage II and stage III disease (P < 0.01), for stage III and stage IV disease (P < 0.05), but not for stage I and stage II disease. According to the histological grading, there was a significant difference between the mean AgNOR number for the well-differentiated and moderately differentiated tumors (P < 0.05), and for the moderately differentiated and poorly differentiated types (P < 0.01). These results suggest that the number of AgNORs in the nucleus is a significant indicator of laryngeal carcinomas.
采用嗜银染色技术，对76例喉鳞状细胞癌手术切除标本中的核仁组织区（NORs）进行了研究。每个细胞核中嗜银核仁组织区（AgNORs）的平均数量为4.3±1.38（标准差）。T1或T2期病变的平均AgNOR数量在统计学上低于T3或T4期病变（P<0.05）。N0期患者的平均AgNOR数量低于N1或N2或N3期肿瘤患者（P<0.05）。II期和III期病变的平均AgNOR数量之间也存在统计学显著差异（P<0.01），III期和IV期病变之间存在统计学显著差异（P<0.05），但I期和II期病变之间无差异。根据组织学分级，高分化肿瘤与中分化肿瘤的平均AgNOR数量之间存在显著差异（P<0.05），中分化与低分化类型之间存在显著差异（P<0.01）。这些结果表明，细胞核中AgNORs的数量是喉癌的一个重要指标。
