Kogoma T, von Meyenburg K
Department of Microbiology, The Technical University of Denmark, Lyngby-Copenhagen.
EMBO J. 1983;2(3):463-8. doi: 10.1002/j.1460-2075.1983.tb01445.x.
The sdrA224 mutants of Escherichia coli K-12, capable of continued DNA replication in the absence of protein synthesis (stable DNA replication), tolerate inactivation of the dnaA gene by insertion of transposon Tn10. Furthermore, oriC, the origin of E. coli chromosome replication, can be deleted from the chromosome of sdrA mutants without loss of viability. The results suggest the presence of a second, normally repressed, initiation system for chromosome replication alternative to the 'normal' dnaA+ oriC+-dependent initiation mechanism.
大肠杆菌K-12的sdrA224突变体能够在缺乏蛋白质合成的情况下持续进行DNA复制（稳定DNA复制），它能耐受因转座子Tn10插入而导致的dnaA基因失活。此外，大肠杆菌染色体复制起点oriC可从sdrA突变体的染色体上删除而不影响其生存能力。这些结果表明，除了“正常的”依赖dnaA+oriC+的起始机制外，还存在第二种通常被抑制的染色体复制起始系统。
